ISOLATED AMPHIBIAN RETINA

孤立的两栖动物视网膜

• 批准号: 6122342
• 负责人: THOMAS G EBREY
• 金额: $ 0.81万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-02-10 至 2000-01-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6122342
• 关键词: Chordata; bioimaging /biomedical imaging; biomedical resource; carbohydrates; eye; human tissue; magnetic resonance imaging; nuclear magnetic resonance spectroscopy; technology /technique

31P and 1H spectra have been obtained on intact isolated and superfused toad retinae. Up to eight retinae are studied in a commercial probe and a single retina is studied in a purpose-built small-sample spectroscopy probe. Adequacy of tissue maintenance conditions has been demonstrated. Differences in phosphorus metabolite concentrations have been observed under conditions of light and dark adaptation. Cyclic GMP has been demonstrated in the 31P NMR spectrum of retina.The Pa/Pi ratio increases and [La] decreases in high calcium solution, a useful finding since calcium is thought to be involved in light adaptation.When the Na/K pump is blocked with ouabain in low calcium, both Pa/Pi and lactate decrease, suggesting differing functional roles for anaerobic and aerobic glycolysis.

在完整隔离和 超级吞噬蟾蜍视网膜。 最多研究了八个视网膜 商业探针和单个视网膜是在专门建造的 小样本光谱探针。 组织维护的充分性 已经证明了条件。 磷的差异 在光条件下已经观察到了代谢物浓度 和黑暗的适应。 循环GMP已在31p NMR中证明 视网膜的频谱。PA/PI比率增加，[la]减少 高钙溶液，这是一个有用的发现，因为钙被认为是 当Na/K泵被阻塞时，参与光适应 在低钙中的ouabain，PA/PI和乳酸降低，表明 厌氧和有氧糖酵解的功能作用不同。