STRUCTURE REFINEMENT OF MUTANT VERSIONS OF E COLI ASPARTATE TRANSCARBAMOYLASE

大肠杆菌天冬氨酸转氨甲酰酶突变体的结构精修

• 批准号: 6221094
• 负责人: EVAN R KANTROWITZ
• 金额: $ 0.13万
• 依托单位: MELLON PITTS CORPORATION (MPC CORP)
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6221094
• 关键词: bacteria; bioengineering /biomedical engineering; biomedical resource; cardiovascular system; infection; oral pharyngeal; proteins

The projects pursued in this laboratory center on the topics of structure/function correlation of T cell receptors, and signal transduction pathways in effector T cells. Several V* genes have been identified which are used to carry out recognition of defined allospecificities. Tumor specific T cell lines which express a particular T cell receptor have been use to identify tumor specific antigens. This has already led to identification of a mucin molecule (human mucin muc-1) expressed by ductal epithelial cells, and by tumors in an altered glycosylation form capable of stimulating T cell immunity. The structure of mucin is being characterized by NMR and circular dichroism in order to select the optimal vaccine or immunotherapeutic antigen for breast and pancreatic cancer. The effector T cells which we study in both systems respond to antigen stimulation by activating several intracellular signaling pathways. We are especially interested in the PKC pathway as a mean of pr omoting and controlling T cell activation.

在这个实验室中心进行的项目有关 T细胞受体的结构/功能相关性和信号 效应T细胞中的转导途径。 几个v*基因已经 确定用于执行定义的识别的确定 高特异性。 表达肿瘤特异性T细胞系 特定的T细胞受体已用于鉴定特异性肿瘤 抗原。 这已经导致粘蛋白分子的鉴定 （人粘蛋白MUC-1）由导管上皮细胞和 以刺激T细胞的糖基化形式改变形式的肿瘤 免疫。 粘蛋白的结构以NMR为特征，并且 圆形二科运动，以选择最佳疫苗或 用于乳腺癌和胰腺癌的免疫治疗抗原。 这 我们在两个系统中研究的效应T细胞对抗原有反应 通过激活几个细胞内信号通路来刺激。 我们对PKC途径特别感兴趣 旋转和控制T细胞激活。