MS & MS ANALYSIS OF MALDI GENERATED IONS OF CHARGE DERIVATIZED PEPTIDES

多发性硬化症

• 批准号: 6258780
• 负责人: JOHN ALLISON
• 金额: $ 0.36万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6258780
• 关键词: biological products; biomedical equipment development; biomedical resource; biotechnology; proteins; technology /technique development

The utility of charge-derivatized peptides has been a substantial development from this Facility, first in the context of FAB analyses, and more recently in the context of MALDI. It was unexpected that charge-localized peptides respond well in MALDI. The mechanism of ionization in this experiment is not well-understood, but is presumably similar to the mechanism through which other cations such as sodium ions are formed. As in the case of protonated peptides, charge-derivatized peptides form only a single peak in a MALDI mass spectrum using a linear time-of-flight mass spectrometer. However, their MS/MS spectra (Post-Source Decay mass spectra) are very different from those for protonated peptides. For many peptides, the charge-localized derivatives form predominantly a-type fragment ions. Sidechain losses are also observed for some residues, and the mechanism for their formation may be similar to those through which the a-type fragments are formed. The goal of this project is to understand the mechanisms through which fragment ions are formed from charge-derivatized spectra, such that sequence information can be extracted from the PSD mass spectra. Incorporation of continued MALDI advances, such as delayed extraction (DE) is also important for optimizing information obtained from these experiments.

电荷衍生肽的效用已经很重要 该设施的开发，首先是在Fab分析的背景下， 最近在马尔迪的背景下。 意外的是 电荷定位的肽在MALDI中反应良好。 机制 该实验中的电离不是很好地理解，而是 大概与其他阳离子这样的机制相似 由于钠离子形成。 就像质子化肽一样 电荷衍生的肽仅在MALDI质量中形成一个峰 使用线性飞行时间质谱仪的光谱。 然而， 他们的MS/MS光谱（后源衰减质谱）非常 与质子化肽不同。 对于许多肽， 电荷定位的衍生物主要形成A型片段离子。 还观察到某些残基的侧链损失，并且 它们形成的机制可能类似于 形成了A型片段。 这个项目的目标是 了解碎片离子从 电荷衍生的光谱，使得序列信息可以是 从PSD质谱中提取。 加入MALDI 进步（例如延迟提取（DE））对于 优化从这些实验获得的信息。