SEQUENCE ANALYSIS OF CHARGE DERIVATIZED PEPTIDES BY ELECTROSPRAY MASS SPEC

通过电喷雾质谱对电荷衍生化肽进行序列分析

• 批准号: 6258784
• 负责人: JACK T WATSON
• 金额: $ 0.19万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6258784
• 关键词: biological products; biomedical equipment development; biomedical resource; carbohydrates; technology /technique development

Methodology for the preparation and analysis of N-tris(2,4,6-trimethoxyphenyl)phosphonium-acetyl (TMPP-Ac) derivatives of peptides has been developed in this laboratory. Most often we analyze the TMPP-Ac derivatives of peptides by FAB with subsequent CAD or by MALDI with subsequent PSD to obtain sequence information. We are investigating the analysis of the TMPP-Ac derivatives by ESI with in-source fragmentation to obtain sequence information. Our preliminary experiments show that in-source fragmentation of the TMPP-Ac derivatized peptides is sensitive to different parameters such as cone voltage, capillary voltage, nebulizing gas flow rate, etc. The developing methodology is being extended to TMPP-Ac derivatization of peptide fragments from tryptic digests of proteins with subsequent analysis by LC-MS using electrospray.

制备和分析方法 N-三(2,4,6-三甲氧基苯基)乙酰基(TMPP-Ac)衍生物 该实验室已开发出多种肽。 大多数时候我们 通过 FAB 和随后的 CAD 分析肽的 TMPP-Ac 衍生物 或者通过MALDI结合后续的PSD来获取序列信息。 我们 正在研究 ESI 对 TMPP-Ac 衍生物的分析 源内碎片以获得序列信息。 我们的 初步实验表明，源内碎片 TMPP-Ac 衍生肽对不同参数敏感，例如 如锥孔电压、毛细管电压、雾化气体流量等。 开发方法正在扩展到 TMPP-Ac 衍生化 蛋白质胰蛋白酶消化的肽片段，以及随后的 使用电喷雾通过 LC-MS 进行分析。