CORE--TRANSGENIC ANIMAL RESOURCE

核心--转基因动物资源

• 批准号: 6099840
• 负责人: Richard A. Flavell
• 金额: $ 13.33万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6099840
• 关键词: T cell receptor; animal breeding; animal colony; autoimmunity; biomedical facility; gene targeting; genetically modified animals; laboratory mouse

The overall objective of this Program Project is to determine the nature and diversity of mechanism active in the regulation and dysregulation of the immune system in autoimmunities. Such study presupposes that the tools to identify, characterize and manipulate cells, genes and molecules of this system are available to the Program Project. Key tools in this analysis are highly specialized transgenic, mutant, homozygous deficient ("gene knock-out") congenic, recombinant and other mutant mouse strains. These strains provide the key reagents for each of the projects in this Program Project, and the availability of this type of genetically altered reagents for each of the projects in this Program Project, and the availability of this type of genetically altered animal has in general revolutionized the study of immunobiology. These strains will permit clear study of autoimmunity in vivo and in vitro. Key strains will include TCR+ and Ig- transgenic mice to permit direct analysis of autoreactive T and B cells, and "gene knock-out" mice to provide genetic backgrounds deficient in specific significant aspects of immunity. These strains are crucial to our studies of autoimmunity. This core mouse breeding facility is thus an essential resource for the research projects.

该计划项目的总体目标是确定 以及活跃调节和失调的机制的多样性 自身免疫中的免疫系统。此类研究的前提是工具 识别、表征和操纵细胞、基因和分子 该系统可供计划项目使用。这方面的关键工具 分析是高度专业化的转基因、突变、纯合缺陷 （“基因敲除”）同源、重组和其他突变小鼠品系。 这些菌株为本研究中的每个项目提供了关键试剂 计划项目，以及此类转基因药物的可用性 本计划项目中每个项目的试剂，以及 此类转基因动物的可用性总体上 彻底改变了免疫生物学的研究。这些菌株将允许清除 体内和体外自身免疫研究。主要菌株将包括 TCR+ 和 Ig 转基因小鼠，可直接分析自身反应性 T 和 B 细胞和“基因敲除”小鼠以提供遗传背景缺陷 在免疫的特定重要方面。这些菌株对于 我们对自身免疫的研究。因此，这个核心小鼠饲养设施是一个 研究项目的重要资源。