3DBioNet: an integrated technological platform for 3D micro-tissues

3DBioNet：3D微组织集成技术平台

基本信息

批准号：
MR/R025762/1
负责人：
Raphael Levy
金额：
$ 79.77万
依托单位：
University of Liverpool
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2018
资助国家：
英国
起止时间：
2018 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=MR%2FR025762%2F1
关键词：
3DBioNet integrated technological platform 3D

项目摘要

Maybe you have already seen cells under a microscope? Plants, animals and humans are made of cells. For approximately a century, scientists have routinely grown cells on flat transparent surfaces (glass or plastic). Growing cells on flat surfaces is very convenient. In fact, it has been so successful that a lot of what we know about cell components such as the nucleus (which contains the genetic information) and mitonchodria (which provides energy) comes from such experiments; this also includes discovery of drugs and tests of their potential toxicity. However, in real living organisms cells are not attached to a flat surface. Instead, they interact with their three dimensional environment which includes other cells as well as factors secreted by cells. Scientists now realize more and more the importance of this difference between growing cells on a flat surface or growing them in 3D: it modifies their shape, how they communicate with each others and how they respond to changes in their environment. Until recently, animal experiments have been the main alternative to cells grown on flat surfaces. Whilst animal experiments remain essential to our understanding of biology and to the discovery of new drugs, they do not perfectly mimic human biology and it is also desirable to reduce their number for ethical reasons.In the last decade, scientists have started to culture cells in 3D thereby producing micro-tissues, often starting from stem cells. These micro-tissues made of human-derived cells resemble human tissues. But, just like going from 2D printing to 3D printing requires new materials, new software, new ideas and new procedures, going from 2D cell culture to 3D cell culture requires a large number of innovations in the methods, materials and technologies that scientists use to design, perform, analyse and interpret their experiments. The purpose of the 3DbioNet network is to bring together the skills of scientists from many different disciplines within academia and industry needed to identify the stumbling blocks and help building this new way of doing biology.

也许您已经在显微镜下见过细胞？植物、动物和人类都是由细胞组成的。大约一个世纪以来，科学家们通常在平坦的透明表面（玻璃或塑料）上培养细胞。在平坦的表面上培养细胞非常方便。事实上，它是如此成功，以至于我们对细胞成分（例如细胞核（包含遗传信息）和线粒体（提供能量））的了解都来自此类实验；这还包括药物的发现及其潜在毒性的测试。然而，在真实的生物体中，细胞并不附着在平坦的表面上。相反，它们与三维环境相互作用，其中包括其他细胞以及细胞分泌的因子。现在，科学家们越来越认识到在平面上生长的细胞与在 3D 中生长的细胞之间的这种差异的重要性：它改变了细胞的形状、细胞之间的沟通方式以及细胞对环境变化的反应。直到最近，动物实验一直是平面细胞生长的主要替代方案。虽然动物实验对于我们理解生物学和发现新药仍然至关重要，但它们并不能完全模仿人类生物学，而且出于伦理原因，也希望减少动物实验的数量。在过去的十年中，科学家们开始在3D 从而产生微组织，通常从干细胞开始。这些由人类细胞组成的微组织类似于人体组织。但是，就像从 2D 打印到 3D 打印需要新材料、新软件、新想法和新程序一样，从 2D 细胞培养到 3D 细胞培养也需要科学家用来实现的方法、材料和技术的大量创新。设计、执行、分析和解释他们的实验。 3DbioNet 网络的目的是汇集学术界和工业界许多不同学科的科学家的技能，以识别障碍并帮助建立这种新的生物学方法。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Quantification of the nonlinear susceptibility of the hydrogen and deuterium stretch vibration for biomolecules in coherent Raman micro-spectroscopy.

DOI：
10.1002/jrs.6164
发表时间：
2021-09
期刊：
JOURNAL OF RAMAN SPECTROSCOPY
影响因子：
2.5
作者：
Boorman, Dale;Pope, Iestyn;Masia, Francesco;Watson, Peter;Borri, Paola;Langbein, Wolfgang
通讯作者：
Langbein, Wolfgang

An insight into the iPSCs-derived two-dimensional culture and three-dimensional organoid models for neurodegenerative disorders.