CHARACTERIZATION OF SALIVARY ASSOCIATED LYMPHOID TISSUE

唾液相关淋巴组织的特征

• 批准号: 2897017
• 负责人: Paul C. Montgomery
• 金额: $ 22.68万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2897017
• 关键词: cell adhesion; cell motility; cell population study; cholera toxin; cytokine; epithelium; flow cytometry; immunization; immunocytochemistry; immunoglobulin A; immunoregulation; intracellular transport; laboratory mouse; laboratory rabbit; laboratory rat; ligands; lymphocyte; monoclonal antibody; mucosal immunity; phenotype; radioimmunoassay; salivary glands; secretory immune system; starch; tissue /cell culture

The long-term objectives of this project are to characterize the regulatory events affecting lymphoid cell interactions, composition and traffic as well as immune responses within salivary gland tissues. The overall hypotheses to be tested are: that specific epithelial cell adhesion (or ligand) molecules mediate the selective localization of lymphoid populations within individual salivary gland tissues; that delivery of antigen/cytokine signals to specific mucosal inductive sites will potentiate IgA antibody expression in saliva and that the localization of specific lymphoid populations within major salivary glands plays a role in the development of salivary IgA antibody responses. The specific aims are: 1. To isolate and characterize the epithelial cell adhesion (or ligand) molecules mediating lymphocyte retention within salivary gland tissues. This aim will determine the structure of the epithelial cell adhesion (or ligand) molecules from individual salivary glands and characterize the mechanism by which these molecules mediate lymphocyte retention within glandular tissues. 2. To study the effects of mucosal inductive site antigen/cytokine delivery on the potentiation of IgA antibody responses. This aim will test the ability of bioadhesive degradable starch microparticles and the comparative capacity of poly(DL- lactide-co-glycolide) (PLG) and cholera toxin B-PLG microparticles to deliver mucosal inductive site antigen/cytokine signals that will potentiate IgA antibody responses in saliva and individual salivary glands. 3. To determine if the localization of specific lymphoid subpopulations plays a role in the development of IgA antibodies in individual salivary gland tissues. This aim will test the effects of mucosal immunization on glandular lymphocyte distribution and traffic to individual salivary glands. Investigations in the rat model will utilize biochemical and immunological approaches in conjunction with cell and tissue culture systems, motility and adherence assays, cell isolation and flow cytometry as well as in situ and adoptive transfer techniques. These studies will define new strategies which will allow optimal manipulation and utilization of salivary-associated lymphoid components for immune- mediated host defenses in the oral cavity.

该项目的长期目标是表征 影响淋巴样细胞相互作用，组成和 唾液腺组织中的交通和免疫反应。 这 要测试的总体假设是：特定的上皮细胞 粘附（或配体）分子介导选择性定位 单个唾液腺组织中的淋巴样群体；那 将抗原/细胞因子信号传递到特定的粘膜电感位点 将增强唾液中IgA抗体的表达 在主要唾液腺内的特定淋巴样群体的定位 在唾液IgA抗体反应的发展中起作用。 这 具体目的是：1。隔离和表征上皮细胞 粘附（或配体）分子介导淋巴细胞保留率 唾液腺组织。 这个目标将决定 来自单个唾液的上皮细胞粘附（或配体）分子 腺体并表征这些分子介导的机制 腺体组织中的淋巴细胞保留。 2。研究 粘膜电感部位抗原/细胞因子递送在增强的 IGA抗体反应。这个目标将测试生物粘附的能力 可降解的淀粉微粒和聚（DL-的比较能力） 乳酸 - 糖苷）（PLG）和霍乱毒素B-PLG微粒至 提供粘膜诱导部位抗原/细胞因子信号，该信号将 唾液和个体唾液中的增强IgA抗体反应 腺体。 3。确定特定淋巴机的定位是否 亚群在IGA抗体的发展中起作用 单个唾液腺组织。 这个目标将测试 腺体淋巴细胞分布和交通的粘膜免疫 个体唾液腺。 大鼠模型中的调查将使用 生化和免疫学方法与细胞结合和 组织培养系统，运动性和依从性测定，细胞分离和 流式细胞仪以及原位和收养转移技术。这些 研究将定义新策略，以允许最佳操纵 并利用与唾液相关的淋巴成分用于免疫 口腔中介导的宿主防御。