SALIVARY ASSOCIATED LYMPHOID TISSUE

唾液相关淋巴组织

• 批准号: 2130672
• 负责人: Paul C. Montgomery
• 金额: $ 18.1万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 1996-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2130672
• 关键词: acinar cell; antibody formation; cell cell interaction; cell population study; cytokine; electron microscopy; enzyme inhibitors; epithelium; flow cytometry; fluorescence microscopy; immunization; immunoglobulin A; immunoglobulin isotypes; immunoregulation; laboratory rabbit; laboratory rat; leukocyte adhesion molecules; mature animal; newborn animals; phenotype; radioimmunoassay; radiotracer; reagent /indicator; receptor binding; salivary glands; secretory immune system; tissue /cell culture

The long-term objectives of this study are to characterize the cellular components of salivary gland-associated lymphoid tissue and define regulatory factors involved in the appearance, localization and development of immunocompetent cells in salivary glands. The specific aims are: 1. to characterize and determine the origin of lymphoid cells present in salivary glands; 2. to define the mechanisms regulating lymphoid cell localization within salivary glands; and 3. to study the effect of cytokines on the development and function of immunocompetent cells in salivary gland tissues. Experiments will be conducted in the rat model, which has a well-characterized oral mucosal-associated immune system. Flow cytometry will be used to study phenotype expression in cells isolated from 'unimmunized' neonatal and adult major salivary glands. The impact of in vivo antigen perturbation on lymphocyte subset appearance in adult salivary gland tissues will also be assessed. The origin of lymphoid subsets in salivary glands will be studied using in situ labelling and adoptive transfer techniques. Lymphocyte-salivary gland interactions will be characterized for individual major salivary gland types in both adults and neonates using an in vitro binding assay. The phenotypes of adherent cells will also be determined. Inhibitor studies will be used to define the lymphocyte adhesion receptor and the epithelial cell ligand. A salivary gland tissue culture system will be used to assess the effect of IgA-enhancing cytokines on immunoglobulin and antibody production. Based on the in vitro culture data, selected cytokines will be tested for their capacity to enhance IgA immunoglobulin and antibody production in vivo. These effects will be correlated with changes in salivary gland lymphocyte subset distribution in stimulated animals. These studies will provide a more complete understanding of regulatory events governing salivary gland-associated lymphoid components and define new approaches to control mucosal immune responses in the oral cavity.

这项研究的长期目标是表征细胞 唾液腺相关淋巴组织的成分并定义 外观，本地化和 唾液腺中免疫能力细胞的发展。 具体 目的是：1。表征和确定淋巴样细胞的起源 存在于唾液腺中； 2。定义调节的机制 淋巴样细胞在唾液腺内的定位；和3。研究 细胞因子对免疫能力的发育和功能的影响 唾液腺组织中的细胞。 实验将在 大鼠模型，具有良好的口服粘膜相关免疫 系统。 流式细胞仪将用于研究中的表型表达 从“未免疫”新生儿和成人主要唾液中分离出的细胞 腺体。 体内抗原扰动对淋巴细胞子集的影响 成人唾液腺组织中的外观也将被评估。 这 唾液腺中淋巴子群的起源将使用In研究 原位标签和收养转移技术。 淋巴细胞 - 呈水分 腺体相互作用将以单个主要的唾液为特征 成人和新生儿的腺体类型都使用体外结合测定法。 还将确定粘附细胞的表型。 抑制剂 研究将用于定义淋巴细胞粘附受体和 上皮细胞配体。 唾液腺组织培养系统将是 用于评估IgA增强细胞因子对免疫球蛋白的影响 和抗体产生。 根据体外培养数据，选定 细胞因子将测试其增强IGA免疫球蛋白的能力 和体内抗体产生。 这些效果将与 刺激的唾液腺淋巴细胞亚群分布的变化 动物。 这些研究将提供对 控制唾液腺相关淋巴成分的调节事件 并定义了控制口服粘膜免疫反应的新方法 腔。