IN VITRO STUDIES OF HUMAN MESOTHELIAL CELL CARCINOGENESIS

人间皮细胞致癌的体外研究

• 批准号: 3774863
• 负责人: B I GERWIN
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3774863
• 关键词: RNA splicing; carcinogenesis; cell line; disease /disorder model; gene expression; gene induction /repression; genetic promoter element; growth factor receptors; human genetic material tag; human tissue; ligands; mesothelioma; neoplasm /cancer genetics; nucleic acid repetitive sequence; peptides; platelet derived growth factor; receptor expression; tumor antigens

Cell lines from human mesotheliomas have been shown to express the platelet-derived growth factor PDGF-A gene product, while normal human mesothelial cells do not. Production of PDGF-B chain protein was not detected. A model cell was shown to be tumorigenic. This model was created by expressing the long-splice variant of human PDGF-A chain under the control of a Moloney viral LTR promoter in a non-tumorigenic, T-antigen immortalized human mesothelial cell. Analysis of the receptor-ligand interactions in this cell line, human mesothelioma cell lines and normal and T-antigen expressing, non-tumorigenic human mesothelial cells is underway. PDGFR-alpha but not PDGFR-beta expression is down regulated in mesothelioma as compared to normal mesothelial cells.

来自人类间皮瘤的细胞系已被证明表达 血小板源性生长因子PDGF-A基因产物，而正常人 间皮细胞则不然。 PDGF-B链蛋白的产生没有 检测到。模型细胞被证明具有致瘤性。此模型已创建 通过在以下条件下表达人PDGF-A链的长剪接变体 非致瘤性 T 抗原中莫洛尼病毒 LTR 启动子的控制 永生化人类间皮细胞。 受体-配体分析 该细胞系、人间皮瘤细胞系和正常细胞之间的相互作用 表达 T 抗原的非致瘤性人间皮细胞是 进行。 PDGFR-α 表达下调，但 PDGFR-β 表达不下调 间皮瘤与正常间皮细胞相比。