CNS EFFICACY OF ANTIOXIDANTS FROM PLANTS

植物抗氧化剂的中枢神经系统功效

• 批准号: 6019255
• 负责人: WILLIAM C COLE
• 金额: $ 28.88万
• 依托单位: NATURAL PRODUCTS RESEARCH, INC.
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-01 至 2001-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6019255
• 关键词: antioxidants; central nervous system; method development; monoclonal antibody; neurobiology; neuropharmacologic agent; physical chemical interaction; plant extracts

DESCRIPTION (Adapted from Applicant's Abstract): The central nervous system (CNS) appears to be especially vulnerable to oxidative stress which is believed to be one of the major factors in neural degeneration. The reactive oxygen species (ROS) which cause the oxidative stress, have been implicated in the etiology of several CNS malfunctions, including Parkinsonism, Huntington's Disease, Alzheimer's Disease and aging of brain. The formation and spread of ROS may be delayed or inhibited by antioxidants which may be of either endogenous or exogenous origin. A number of substances derived from plants have been shown to possess significant antioxidant activity and, therefore, have potential direct or indirect applications in the treatment of neurodegenerative diseases. In Phase I of our project, we have developed methods for the semipreparative extraction, quantitative determination and in vitro biological assays of five plan antioxidants, viz., beta-carotene from carrots, lutein from parsley, lycopene form tomato paste, and curcumin and turmerin from turmeric. In Phase II of the project, we propose to address the following issues: 1) to develop procedures for the commercial preparation of each of these compounds in pure form and in good yield, using the least expensive instrumentation and technology; 2) to extend the assay of biological activities of individual and mixtures (cocktails) of these antioxidants to in vivo conditions so as to complement the in vitro methods already developed in Phase I; and 3) to develop specific monoclonal antibodies against lutein, curcumin and turmerin. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述（改编自申请人的摘要）：中枢神经 系统（CNS）似乎特别容易受到氧化应激的影响 被认为是神经变性的主要因素之一。 这 导致氧化应激的活性氧（ROS）已经是 与几个中枢神经系统故障的病因有关，包括 帕金森氏症，亨廷顿氏病，阿尔茨海默氏病和衰老 脑。 ROS的形成和传播可能会被延迟或抑制 可能具有内源性或外源性的抗氧化剂。 一个 已显示出植物的物质数量已显示 显着的抗氧化活性，因此具有潜在的直接或 间接应用神经退行性疾病。 在 我们项目的第一阶段，我们已经开发了半程制作的方法 提取，定量确定和体外生物学测定 五种计划抗氧化剂，即来自胡萝卜的β-胡萝卜素，叶黄素， 欧芹，番茄红素形成番茄酱，姜黄素和Turmerin 姜黄。 在该项目的第二阶段，我们建议解决以下 问题：1）制定每种商业准备程序的程序 这些化合物以纯形式和良好的产量使用 昂贵的仪器和技术； 2）扩展测定 这些个人和混合物（鸡尾酒）的生物学活动 体内条件的抗氧化剂，以补充体外 已经在第一阶段开发的方法； 3）开发特定 抗叶黄素，姜黄素和Turmerin的单克隆抗体。 拟议的商业应用程序：不可用