IR MALDI ION TRAP MASS SPECTOMETER FOR DNA ANALYSIS

用于 DNA 分析的 IR MALDI 离子阱质谱仪

• 批准号: 2829993
• 负责人: Vladimir M Doroshenko
• 金额: $ 9.96万
• 依托单位: SCIENCE AND ENGINEERING SERVICES, INC.
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至 2000-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2829993
• 关键词: biomedical equipment development; electrospray ionization mass spectrometry; infrared spectrometry; lasers; matrix assisted laser desorption ionization; nucleic acid quantitation /detection; nucleic acid sequence

DESCRIPTION : (Adapted from that applicant's abstract) Mass spectrometric DNA analysis has a great potential since it eliminates time-consuming stages of DNA reading by traditional methods of gel electophoresis and fluorescent analysis . It is proposed to develop a matrix-assisted laser desorption/ionization (MALDI) ion trap mass spectrometer (ITMS) for DNA analysis. The presence of fragments is very common in MALDI spectra of biomolecules that makes analysis of even simple DNA mixtures extremely complicated especially when automated systems are considered. In contrast to the time-of-flight (TOF) mass spcetrometer (MS) commonly used with MALDI ion sources, this proposal will improve the yield of the molecular DNA ions in the MALDI process, increase the sensitivity and reliabilility of the method, and allow automation of DNA MS analysis. In Phase I, an ITMS driven at low frequency to operate in the range up to m/z 30,000 will be built and interfaced with MALDI ion source without using strong electric fields for extracting ions. A tunable infrared laser, which showed good results in obtaining mass spectra with minimum fragmentation in the experiments of TOF-MS, will be utilized in the MALDI ion source. In Phase II, an automated system for high throughput DNA analysis will be built using a cooled 2D chip sample array probe. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述：（根据该申请人的摘要改编）质谱DNA 分析具有很大的潜力，因为它消除了DNA的耗时阶段 通过传统的凝胶电疗法和荧光分析的读数。 提议开发基质辅助激光解吸/电离（MALDI） 离子陷阱质谱仪（ITM）用于DNA分析。碎片的存在 在生物分子的MALDI光谱中非常常见，该光谱使得分析甚至 简单的DNA混合物极为复杂，尤其是自动化系统 经过考虑的。与飞行时间（TOF）质量旋转仪（MS）相反 该提案通常与马尔丁离子来源一起使用，将提高 MALDI过程中的分子DNA离子，提高灵敏度和 该方法的可靠性，并允许自动化DNA MS分析。在阶段 I，以低频驱动的ITM在最高M/z 30,000的范围内运行 将在不使用强度的情况下与Maldi Ion源建立并与Maldi Ion源连接 用于提取离子的电场。可调红外激光，显示 在获得最小碎片中获得质谱的良好结果 TOF-MS的实验将用于MALDI离子源。在第二阶段， 将使用用于高吞吐量DNA分析的自动化系统 冷却的2D芯片样品阵列探针。 拟议的商业应用程序：不可用