Quadrupole Ion Trap/FTICR Tandem Mass Spectrometer

四极杆离子阱/FTICR 串联质谱仪

• 批准号: 7294292
• 负责人: Vladimir M Doroshenko
• 金额: $ 36.68万
• 依托单位: SCIENCE AND ENGINEERING SERVICES, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-15 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7294292
• 关键词: Address; Amino Acid Sequence; Amino Acids; Area; Atmospheric Pressure; Biochemical; Biological; Biomedical Research; Buffers; Cells; Cleaved cell; Clinical Research; Cyclotrons; DNA Sequence Rearrangement; Detection; Dissociation; Drug Industry; Due Process; Electrons; Electrospray Ionization; Fourier Transform; Frequencies; Gases; Generations; Goals; Helium; Hybrids; Ions; Isoleucine; Laboratories; Leucine; Marketing; Mass Fragmentography; Mass Spectrum Analysis; Methods; Modification; Molecular; Molecular Weight; Nature; Object Attachment; Oligosaccharides; Operative Surgical Procedures; Pattern; Peptide Sequence Determination; Peptides; Performance; Phase; Phase I Clinical Trials; Phosphorylation; Post-Translational Modification Site; Post-Translational Protein Processing; Principal Investigator; Problem Solving; Process; Property; Proteins; Purpose; Radio; Range; Reaction; Resolution; Site; Small Business Funding Mechanisms; Small Business Innovation Research Grant; Source; Spatial Distribution; Spectrometry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Structure; System; Techniques; Technology; Time; Translations; United States National Institutes of Health; Vertebral column; Work; analytical method; base; biological research; carboxylation; commercialization; concept; cost; design; glycosylation; instrument; ionization; ionization technique; magnetic field; mass analyzer; mass spectrometer; novel; pressure; programs; protein aminoacid sequence; research study; size; sulfation; tandem mass spectrometry; tool

DESCRIPTION (provided by applicant): The purpose of this proposal is to develop a novel tandem mass spectrometry (or MS/MS) technology for mass and structural analysis of biomolecules that could combine excellent ion isolation and fragmentation capabilities of a quadrupole ion trap (QIT) and high resolution and mass accuracy of Fourier transform (FT) ion cyclotron resonance (ICR) mass spectrometry (MS). The main feature of this technology is the use of a permanent magnet for generation of magnetic field in ICR part of the proposed instrument that will make it cost-efficient, compact in size, and affordable for many applications. The major emphasis is made on producing a homogeneous magnetic field in a maximum space volume that to a certain extent can compensate limitations imposed by the use of a lower magnetic field generated by a permanent magnet in the ICR cell (about 1-1.5 Tesla compared to 7-12 Tesla generated by superconductive magnets). A new permanent magnet design is suggested to achieve this goal. The new instrument is expected to have size, cost and MS/MS capabilities of commercial QIT-MS systems and the resolving power and mass accuracy exceeding that of a popular hybrid quadrupole/time-of-flight (QqTOF) MS.

DESCRIPTION (provided by applicant): The purpose of this proposal is to develop a novel tandem mass spectrometry (or MS/MS) technology for mass and structural analysis of biomolecules that could combine excellent ion isolation and fragmentation capabilities of a quadrupole ion trap (QIT) and high resolution and mass accuracy of Fourier transform (FT) ion cyclotron resonance (ICR) mass spectrometry (MS).该技术的主要特征是在拟议仪器的ICR部分中使用永久磁铁来生成磁场，这将使其具有成本效益，尺寸紧凑，并且对于许多应用而言负担得起。主要的重点是在最大空间体积中产生同质磁场，在一定程度上可以补偿通过使用ICR电池中永久磁体产生的较低磁场所施加的局限性（大约1-1.5 Tesla（大约1-1.5 Tesla），而超导磁体产生的7-12特斯拉（7-12））。建议采用新的永久磁铁设计来实现此目标。预计新仪器将具有商业QIT-MS系统的尺寸，成本和MS/MS/MS/MS/MS/MS/MS/MS功能，并且可以超过流行的混合四倍体/飞行时间（QQTOF）MS的解决功率和质量精度。