BRAIN/GUT AXIS--METHODS TO EXAMINE PEPTIDE DYNAMICS

脑/肠轴——检查肽动力学的方法

• 批准号: 2777560
• 负责人: ROBERT L STEPHENS
• 金额: $ 4.53万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2777560
• 关键词: brain mapping; dorsal motor nucleus; dorsal raphe nucleus; gastrointestinal function; high performance liquid chromatography; microdialysis; neuropeptides; neuroregulation; neurotransmitter transport

Neuropeptides are chemical messengers whose function in the CNS remain unclear. The understanding of physiologic functions of neuropeptides would be greatly augmented by methods allowing sensitive, local analysis of multiple peptide systems over a significant time course. This information, taken together with data from classical (amine, amino acid) neuromodulator systems can provide critical information regarding interactive effects of chemical messengers acting at the DVC. Understanding peptide function and their interaction with aminergic systems provides the neurophysiologic template to understanding gastrointestinal pathologies related to cerebral systems (emotional agitation, cold exposure, surgery, trauma, sepsis, burns and functional bowel disorders), and could impact future therapeutic strategies. Future aims of the investigator include the application of the technology learned with the Pittsburgh groups to the study of conscious animal models, where behavioral influences may be studied. Implantations of chronic indwelling medullary recording arrays is routinely performed in an adjoining laboratory (Dr. Joe Travers-Ohio State). Adaptation of microdialysis technology to these elegant models is a research goal.

神经肽是化学信使，其在中枢神经系统中的功能尚不清楚。 通过允许在重要的时间过程中对多个肽系统敏感的局部分析的方法，可以大大增强对神经肽生理功能的理解。 这些信息与来自经典（胺，氨基酸）神经调节剂系统的数据一起提供了有关在DVC上作用的化学信使的交互作用的关键信息。 了解肽功能及其与AMINEGIC系统的相互作用提供了神经生理模板，以了解与大脑系统有关（情绪激动，冷暴露，手术，创伤，败血症，灼伤和功能性肠道疾病）的胃肠道病理学，并可能影响未来的治疗策略。研究人员的未来目标包括将匹兹堡小组学习的技术应用于有意识的动物模型的研究，在这些动物模型中可以研究行为影响。 慢性留置式记录阵列的植入通常在相邻的实验室（Joe Travers-Ohio State博士）进行。 将微透析技术适应这些优雅的模型是一个研究目标。