GASTRIC FUNCTION--TRH AND 5HT IN CAUDAL RAPHE CONTROL

胃功能--尾中缝控制中的TRH和5HT

• 批准号: 2016746
• 负责人: ROBERT L STEPHENS
• 金额: $ 16.13万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-15 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2016746
• 关键词: laboratory rat; medulla oblongata; membrane potentials; neuroendocrine system; serotonin; serotonin inhibitor; serotonin receptor; stomach motility /pressure; thyrotropin releasing hormone; vagus nerve

DESCRIPTION: TRH and 5HT are colocalized in the projections of caudal raphe nuclei onto dorsal vagal complex (DVC) nuclei. TRH and 5HT synergism appears to be involved in the vagal control of gastric function. Microinjections of selective 5HT antagonists will be performed in DVC in order to identify the 5HT receptor subtype involved in the synergistic response. Serotonin release in the dorsal medulla of fed and fasting animals will be compared to document that serotonin release by caudal raphe nuclei projections plays a key role in enhanced acid secretion and motility after a meal. A possible role of excitatory amino acids and substance P as neurotransmitters in the vagal afferents which mediate gastric stimulatory response after a meal will be examined with the use of the agonists and the antagonists of substance P and glutamate. Studies will also be performed to identify DVC neurons (using extracellular recordings) which are activated by gastric distension. Effects and synergistic interactions of TRH and 5HT microinjection directly onto solitary nucleus or DVC nuclei on the "gastric responsive DVC neurons" will be examined.

描述：TRH和5HT在尾骨的预测中进行了共定位 raphe核对背走迷走神经复合物（DVC）核。 TRH和5HT 协同作用似乎参与了胃的迷走神经控制 功能。将进行选择性5HT拮抗剂的显微注射 在DVC中以识别参与的5HT受体亚型 协同响应。 在美联储背侧髓质中释放5-羟色胺 和禁食动物将与5-羟色胺释放的文献进行比较 尾raphe核的投影在增强酸中起着关键作用 一顿饭后的分泌和运动。 兴奋的可能作用 迷走神经传入中的氨基酸和物质P作为神经递质 将检查哪些介导胃刺激反应 使用激动剂和物质的拮抗剂P和 谷氨酸。 还将进行研究以识别DVC神经元（使用 细胞外记录）通过胃扩张激活。 TRH和5HT显微注射的效果和协同相互作用 直接进入“胃反应的孤立核或DVC核” DVC神经元将进行检查。

项目成果

5-CT or DOI augments TRH analog-induced gastric acid secretion at the dorsal vagal complex.

5-CT 或 DOI 增强 TRH 类似物诱导的背侧迷走神经复合体胃酸分泌。

• DOI: 10.1152/ajpregu.1997.273.5.r1607
• 发表时间: 1997
• 期刊: The American journal of physiology
• 作者: Varanasi,S;Chi,J;StephensJr,RL
• 通讯作者: StephensJr,RL

Stimulation of the nucleus raphe obscurus produces marked serotonin release into the dorsal medulla of fed but not fasted rats--glutamatergic dependence.

刺激中缝暗核会产生显着的血清素释放到进食但未禁食的大鼠的背髓质中——谷氨酸能依赖性。

• DOI: 10.1016/0006-8993(95)00505-k
• 发表时间: 1995
• 期刊: Brain research
• 影响因子: 2.9
• 作者: Mohammed,JR;Saska,TA;Chi,J;StephensJr,RL
• 通讯作者: StephensJr,RL

Methiothepin but not methysergide antagonizes TRH effects at the dorsal vagal complex.

甲硫替平（而非美西麦角）拮抗背侧迷走神经复合体的 TRH 作用。

• DOI: 10.1111/j.1749-6632.1998.tb10231.x
• 发表时间: 1998
• 期刊: Annals of the New York Academy of Sciences
• 影响因子: 5.2
• 作者: Varanasi,S;Chi,J;Rogers,RC;StephensJr,RL
• 通讯作者: StephensJr,RL

Methiothepin attenuates gastric secretion and motility effects of vagal stimulants at the dorsal vagal complex.

甲硫替平减弱迷走神经刺激物对背侧迷走神经复合体的胃分泌和运动作用。

• DOI: 10.1016/s0014-2999(01)01579-5
• 发表时间: 2002
• 期刊: European journal of pharmacology
• 影响因子: 5
• 作者: Varanasi,Sridhar;Chi,Jinhan;StephensJr,RobertL
• 通讯作者: StephensJr,RobertL