MICA: High speed, high resolution imaging of excitable cell networks

MICA：可兴奋细胞网络的高速、高分辨率成像

• 批准号: MR/K015877/1
• 负责人: Tony Wilson
• 金额: $ 179.27万
• 依托单位: University of Oxford
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FK015877%2F1
• 关键词: MICA; speed; resolution; imaging; excitable

The heart and brain function through the fast propagation of electrical impulses. Heart arrhythmias and epileptic seizures are the results of massive electrical surges in these organs, but all cardiac and neurological dysfunction results ultimately from alterations in the patterns of biological electrical activity. Visualising activity in cardiac and neuronal tissue is a fundamental challenge for biomedicine, as each brief electrical signal propagates rapidly over large distances. For example, a single heartbeat involves choreographed waves of electrical activity pervading every cell in the organ. During the same time period, electrical signals in the nervous system can be used to detect the light reflected from an incoming cricket ball, calculate its trajectory, and send the motor commands to make the catch. Our proposal brings together two new techniques in optical engineering that will enable us to capture these rapid events in electrically excitable tissues.This proposal will create a high speed imaging facility, which will enable the wider application of these cutting-edge approaches to studying cardiac and neurological disease.

通过快速传播电脉冲的心脏和大脑功能。心脏心律不齐和癫痫发作是这些器官中大量电潮的结果，但是所有心脏和神经功能障碍最终都来自生物电活动模式的改变。可视化心脏和神经元组织中的活性是生物医学的基本挑战，因为每个简短的电信号在大距离内都会迅速传播。例如，单个心跳涉及遍布器官中每个细胞的电动活动的编排波。在同一时期，神经系统中的电信号可用于检测从传入的板球反射的光，计算其轨迹并发送电动机命令以使捕获。我们的建议汇集了光学工程中的两种新技术，这将使我们能够在电动组织中捕获这些快速事件。该提案将创建高速成像设施，这将使这些尖端方法更广泛地应用心脏和神经疾病。

项目成果

Hypertension-induced remodelling: on the interactions of cardiac risk factors.

• DOI: 10.1113/jp273043
• 发表时间: 2017-06-15
• 期刊: The Journal of physiology
• 作者: Tomek J;Bub G
• 通讯作者: Bub G

Optical control of excitation waves in cardiac tissue.

• DOI: 10.1038/nphoton.2015.196
• 发表时间: 2015-12
• 期刊: Nature photonics
• 影响因子: 35
• 作者: Burton RA;Klimas A;Ambrosi CM;Tomek J;Corbett A;Entcheva E;Bub G
• 通讯作者: Bub G

Macro-micro imaging of cardiac-neural circuits in co-cultures from normal and diseased hearts.

正常和患病心脏共培养物中心脏神经回路的宏观显微成像。

• DOI: 10.1113/jphysiol.2014.285460
• 发表时间: 2015
• 期刊: The Journal of physiology
• 作者: Bub G

Bringing the living brain into focus

让活的大脑成为焦点

• DOI: 10.1038/nphoton.2015.3
• 发表时间: 2015
• 期刊: Nature Photonics
• 影响因子: 35
• 作者: Corbett A

Quantifying distortions in two-photon remote focussing microscope images using a volumetric calibration specimen.

• DOI: 10.3389/fphys.2014.00384
• 发表时间: 2014
• 期刊: Frontiers in physiology
• 影响因子: 4
• 作者: Corbett AD;Burton RA;Bub G;Salter PS;Tuohy S;Booth MJ;Wilson T
• 通讯作者: Wilson T