HOST GENETIC FACTORS AFFECTING SPECIFICITY ON THE COCCIDIA OF SMALL MAMMALS (MYOM

影响小型哺乳动物球虫（MYOM）特异性的宿主遗传因素

• 批准号: 3877451
• 负责人: TERRY L YEATES
• 金额: --
• 依托单位: UNIVERSITY OF NEW MEXICO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3877451
• 关键词: Carnivora; animal population genetics; biological polymorphism; chromosomes; coccidiosis; gene expression; gene frequency; genotype; host organism interaction; karyotype; leishmaniasis; liver cells; microorganism genetics; species difference; trypanosomiasis

This specific objectives of this subproject are to (1) characterize the extent and nature of chromosomal and genic variability in populations of certain rodent and insectivore species; (2) identify the coccidian parasite faunas of these host species; (3) attempt to correlate speciation of the coccidian genus Eimeria with genetic variation and/or ecological distribution patterns of the host populations; and (4) over the long term, use the karyotypic and electrophoretic data on host populations to investigate the role that various loci may play in susceptibility of these hosts to their specific parasite faunas. Our hypothesis is that host specificity and evolution in the coccidian genes Eimeria are determined by host genetics and distribution patterns. Data from previous studies in squirrels, cricetid rodents, moles, shrew- moles, and bats are beginning to support these ideas and during the next four years we will continue to analyze tissue, chromosome and parasite samples from hosts that were collected from 1981 through 1986 as well as collect more hosts and parasites from around the world. Once all the specific pieces of data have been individually itemized (chromosomes counted and banded; gels run and enzyme variation qualified and quantified; parasites measured, identified and described) we should begin to realize the specific objectives listed above. One interesting development that has occurred recently involves Dr. Yates' new research in the South American country of Bolivia. This project, funded by NSF, is generating large samples of mammals and parasites that will allow independent tests of our ideas from Asian and North American species. It may turn out that one of the most immediate applications of our findings will be in South America where parasite diseases are a major health problem. We are currently establishing convenios with the two major tropical disease centers in Bolivia. If successful, this should provide major positive opportunities for use and our MBRS students.

该子项目的具体目标是 (1) 表征 染色体和基因变异的程度和性质 某些啮齿动物和食虫动物种群； (2) 识别 这些寄主物种的球虫寄生动物群； (3)尝试 将球虫属艾美耳球虫的物种形成与遗传联系起来 宿主的变异和/或生态分布模式 人口； (4) 从长远来看，使用核型和 宿主群体的电泳数据以研究其作用 不同的位点可能在这些宿主的易感性中发挥作用 他们特定的寄生虫动物群。 我们的假设是宿主 艾美球虫球虫基因的特异性和进化是 由宿主遗传学和分布模式决定。 数据来自 先前对松鼠、仓鼠、鼹鼠、鼩鼱的研究 鼹鼠和蝙蝠开始支持这些想法，并且在 未来四年我们将继续分析组织、染色体和 从 1981 年至 1986 以及从周围收集更多的宿主和寄生虫 世界。 一旦所有特定的数据都被单独 逐项（染色体计数和条带；凝胶运行和酶 对变化进行定性和量化；测量、鉴定寄生虫 并描述）我们应该开始实现具体目标 上面列出的。 最近发生的一项有趣的进展涉及博士。 耶茨在南美国家玻利维亚进行的新研究。 这 该项目由美国国家科学基金会资助，正在生成大量哺乳动物样本 寄生虫将允许我们在亚洲独立测试我们的想法 和北美物种。 结果可能是最 我们的研究结果将立即应用于南美洲 寄生虫病是一个主要的健康问题。 我们是 目前与两大热带地区建立了协定 玻利维亚的疾病中心。 如果成功，这应该提供 主要积极的使用机会和我们的 MBRS 学生。