REGULATION OF PROTEIN SYNTHESIS MODULATION BY TRNA

TRNA 对蛋白质合成的调控

• 批准号: 3877345
• 负责人: GRACIELA C CANDELAS
• 金额: --
• 依托单位: UNIVERSITY OF PUERTO RICO RIO PIEDRAS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3877345
• 关键词: cell free system; eukaryote; gene expression; genetic regulation; genetic transcription; genetic translation; nucleic acid sequence; protein biosynthesis; transfer RNA

Synthesis of a secretory protein and the points at which the process may be regulated are being investigated using a pair of spider fibroin glands as a model system. The two outstanding characteristics of the system are that the glands can be cultured in vitro, and that through simple manipulations their synthetic activity may be virtually turned off, and then stimulated into a massive production of the protein product. Having characterized the gland, its product and also the template molecule, we have, thus far, been elucidated. All of them exert their regulation on a translational level, one at elongation, the other at the readout now to move on in our characterization, into posttranscriptional regulation. This is justified, because within an early transient wave of macromolecular syntheses preceding the template production, di novo synthesis of small nuclear RNAs and positive strong signals for a U1 RNA probe have been both detected in the stimulated glands. These results indicate that within the orchestration of fibroin production, the small RNAs play a vital role. Small RNAs are currently in the limelight of gene expression because of their proven role(s) in the postranscriptional processing of transcripts in eukaryotes. We, therefore, aim to characterize the small RNAs, particularly the U ones, because of their well established roles as transcript maturators. In view of our preliminary observations, we will explore the system for tissue specific expressions of these molecules, and establish kinetic correlations between selective U gene expressions and elicited fibroin synthesis.

分泌蛋白的合成和 可能正在使用一对 蜘蛛纤维素腺作为模型系统。 两个杰出 系统的特征是可以培养腺体 在体外，通过简单的操纵它们的合成 活动几乎可以关闭，然后刺激成一个 蛋白质产物的大量生产。 表征了 腺体，其产物以及模板分子，我们有 到目前为止，已经阐明了。 他们所有人都对 翻译级别，一个在伸长率上，另一个在读数中 现在继续我们的特征，转录后 规定。 这是有道理的，因为在早期的短暂性中 在模板生产之前的大分子合成浪潮， 小型核RNA和阳性强信号的di Novo合成 对于U1 RNA探针，均已在刺激中检测到 腺体。 这些结果表明在编排 纤维蛋白产生，小型RNA起着至关重要的作用。 小rnas 由于它们 在成绩单的三一笔处理中久经考验的作用 在真核生物中。 因此，我们旨在表征小的RNA， 特别是U的角色，因为它们的角色良好 作为成绩单成熟者。 鉴于我们的初步观察 我们将探索这些系统特定表达的系统 分子，并在选择性U之间建立动力学相关性 基因表达和引起纤维蛋白合成。