FACSTAR PLUS CELL SORTER

FACSTAR PLUS 细胞分选仪

• 批准号: 3521039
• 负责人: ROBERT A HYMAN
• 金额: $ 37万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-03-15 至 1992-03-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521039
• 关键词: biomedical equipment purchase; cell population study; flow cytometry

Flow cytometry has many applications in biology. Acquisition of a FACStarPLUS Cell Sorter for the Flow Cytometry Laboratory at The Salk Institute is proposed to support projects studying: 1) the mechanism of gene amplification in tumor cells, 2) the analysis of hematopoietic cell lineages in transgenic mice, 3) the study of thymocyte progenitors, 4) the isolation and study of hematopoietic stem and progenitor cells, 5) the study of lymphocyte surface antigen function, 6) the regulation of lymphocyte surface antigen expression, 7) the mechanisms by which oncogenes regulate cell growth, and 8) the mapping of human chromosomes. The Salk Institute Flow Cytometry Laboratory comprises a cell sorter and a flow microfluorimeter. The existing cell sorter uses basic technology that was developed in the mid-1970's and no longer meets the need for a machine that can sort cells stained with multiple fluorescent probes with high speed and good purity, especially in situations where the input cells express a wide range of fluorescent intensities, the input sample size is small, non-rectangular sorting gates are required, or isolation of the sorted population as single cell cones or in microcultures is desired. The existing sorter is unique in design and cannot be easily upgraded to meet these needs. It can, however, be optimized for sorting chromosomes, an application that requires considerable sorter time and thus impacts the ability of the Flow Cytometry Laboratory to meet the needs of users whose sorting applications involve problems in cell biology. It is proposed to dedicate the existing sorter to chromosome sorting and to acquire a new sorter that will meet the needs of those who wish to apply cell sorting and multiparameter fluorescent analysis to problems of cell biology.

流式细胞术在生物学中有许多应用。 收购一个 适用于索尔克流式细胞术实验室的 FACStarPLUS 细胞分选仪 建议研究所支持项目研究：1） 肿瘤细胞基因扩增，2）造血细胞分析 转基因小鼠的谱系，3) 胸腺细胞祖细胞的研究，4) 造血干细胞和祖细胞的分离和研究，5) 研究淋巴细胞表面抗原功能，6）调节 淋巴细胞表面抗原表达，7）癌基因的机制 调节细胞生长，8) 人类染色体图谱。 索尔克研究所流式细胞术实验室包括细胞分选仪和 流动微量荧光计。 现有的细胞分选仪使用的基本技术是 于 1970 年代中期开发，已不再满足机器的需求 可以对用多种荧光探针染色的细胞进行高分选 速度和良好的纯度，特别是在输入细胞的情况下 表达宽范围的荧光强度，输入样本大小为 需要小的、非矩形的分拣门，或者隔离 需要将群体分类为单细胞锥或微培养物。 这 现有的分拣机设计独特，无法轻易升级以满足 这些需求。 然而，它可以针对染色体排序进行优化， 需要大量分拣机时间并因此影响 流式细胞术实验室满足用户需求的能力 分选应用涉及细胞生物学中的问题。 建议 将现有分选机专用于染色体分选并获取新的 分选机将满足那些希望应用细胞分选的人的需求 针对细胞生物学问题的多参数荧光分析。