Cytomation MoFlo High Performance Flow Cytometer

Cytomation MoFlo 高性能流式细胞仪

• 批准号: 6440929
• 负责人: HERBERT KIM LYERLY
• 金额: $ 44.12万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440929
• 关键词: biomedical equipment purchase; cell population study; clinical biomedical equipment; flow cytometry

DESCRIPTION (provided by applicant): The Molecular Therapeutics Program at Duke University Medical Center (DUMC) is focused on developing cellular therapies and clinical studies of cellular reconstitution. This group of NIH supported investigators within the Departments of Medicine, Pathology, Pediatrics and Surgery is requesting a Cytomation MoFlo high-speed sorter to serve as a dedicated clinical sorter for clinical trials of cellular therapies and for clinical sample analysis. A single high-speed cell sorter is currently available at Duke, but no sorter is dedicated to clinical use for the generation of Good Manufacturing Practice (GMP) grade cellular products required by this group of investigators who will require approximately 95 percent of the total usage of the instrument. In addition, the high-speed sorter would be dedicated to analyze samples from clinical trials. Access to the instrument for non-GMP manufacturing or non-clinical trial analysis will be made available to other users based upon the advice of an internal advisory committee. The instrument will be integrated into the core facilities of the Duke Comprehensive Cancer Center and Duke General Clinical Research Center. Continued financial support for the operation and maintenance of the instrument will be provided by the Duke Comprehensive Cancer Center, the Duke General Clinical Research Center, and a charge-back mechanism. Dr. Lyerly currently runs the GMP facility and will provide oversight. Mr. Cook, who will do the sorting, has 7 years of experience. The instrument will have a single, well-trained operator. In summary, the Molecular Therapeutics Program at DUMC has a demonstrated need and is requesting support for a state-of-the-art Cytomation MoFlo flow cytometer primarily for dedicated GMP clinical cell sorting. Advisory committees, institutional support, financial support for continued maintenance and management plans are in place to insure that the instrument will be fully and appropriately utilized.

描述（由申请人提供）：杜克大学分子治疗项目 大学医学中心 (DUMC) 专注于开发细胞疗法 和细胞重建的临床研究。 NIH 支持的这组 医学、病理学、儿科和 手术室需要一台 Cytomation MoFlo 高速分拣机作为 专用临床分选机，用于细胞疗法的临床试验和 临床样本分析。目前单一高速细胞分选仪 杜克大学有，但没有专门用于临床用途的分选机 生产良好生产规范 (GMP) 级细胞产品 这组调查人员需要大约 95 仪器总使用量的百分比。此外，高速 分选机将专门用于分析临床试验中的样本。访问 用于非 GMP 生产或非临床试验分析的仪器将是 根据内部咨询的建议提供给其他用户 委员会。该仪器将集成到该中心的核心设施中 杜克综合癌症中心和杜克普通临床研究中心。 为仪器的运行和维护提供持续的财政支持 将由杜克大学综合癌症中心、杜克大学总医院提供 临床研究中心，并设有退款机制。莱尔利博士目前 运营 GMP 设施并提供监督。库克先生将负责 分拣，有7年经验。该仪器将有一个， 训练有素的操作员。总之，DUMC 的分子治疗项目 有明确的需求并请求支持最先进的 Cytomation MoFlo 流式细胞仪主要用于专用 GMP 临床细胞 排序。咨询委员会、机构支持、财政支持 持续的维护和管理计划到位，以确保 仪器将得到充分、适当的利用。