ENERGETIC AND STOICHIOMETRIC RELATIONSHIPS INVOLVING RESPIRATION, H, AND ATP

涉及呼吸、H 和 ATP 的能量和化学计量关系

• 批准号: 3857973
• 负责人: R W HENDLER
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3857973
• 关键词: Micrococcaceae; Xenopus; adenosine triphosphate; antibacterial agents; cell biology; cellular respiration; chemical binding; cytochrome oxidase; electron transport; fiber optics; fluorescent dye /probe; laboratory rat; liposomes; mitochondria; oxidative phosphorylation; peptides; stoichiometry

It is currently thought that subunit III of cytochrome oxidase plays an important role in the ability of this enzyme to pump protons. We have shown, however, that this subunit is neither required nor beneficial to this process. Using a rat liver submitochondrial system, the quantitative effect of delta-psi on energies of activation for the synthesis and hydrolysis of free ATP from free ADP and Pi was determined. It was found that approximately 83% of the protonmotive input energy was used to lower the energy of activation for synthesis and approximately 17% to raise the energy of activation for hydrolysis.

目前认为细胞色素氧化酶III亚基在 这种酶泵送质子的能力发挥着重要作用。 我们有 然而，表明该亚基既不是必需的，也无益于 这个过程。 使用大鼠肝脏亚软骨系统，定量影响 δ-psi 对合成和水解活化能的影响 测定游离 ADP 和 Pi 中的游离 ATP。 结果发现 大约 83% 的质子输入能量用于降低 合成活化能约17%，以提高 水解的活化能。