PHYSICOCHEMICAL & BIOANALYTICAL STUDIES USING MLC

物理化学

• 批准号: 3466425
• 负责人: MORTEZA G KHALEDI
• 金额: $ 14.27万
• 依托单位: NORTH CAROLINA STATE UNIVERSITY RALEIGH
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-04-01 至 1994-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3466425
• 关键词: bioassay; biochemistry; bioenergetics; chromatography; drug design /synthesis /production; environmental contamination; hydropathy; organic chemicals; solute; toxicology

The purpose of this work is to explore the highly promising capabilities of Micellar Liquid Chromatography as a powerful technique for chemical analysis and physico-chemical studies in biomedical research. The proposal involves a systematic investigation of the use of Micellar LC for: characterization of physio-chemical properties of biologically important molecules and environmental pollutants; the study of the energetics and mechanisms of solute-micelle interactions and their effects on retention and selectivity in Micellar LC, and the development of rapid and sensitive methods for the analysis of biochemical compounds. A total of six projects are proposed in these three main areas which are of much current interest due to their intrinsic significance, and because of their impact on a broad range of disciplines other than separation sciences; from pharmaceutical chemistry to biotechnology. In the first part, the aim is to apply micellar LC for the quantitation of hydrophobic character of organic compounds. Characterization of hydrophobicity has both a diagnostic and predictive value in various disciplines such as drug design, toxicology, and environmental monitoring. Micellar LC offers clear advantages over both classical methods and RPLC with traditional binary solvents for estimation of hydrophobicity. In the second part of this study, the aim is to better define the physico-chemical basis of retention and selectivity in micellar LC through a careful design of experiments to highlight the significance of solute-micelle interactions. Finally, in the third part, the aim is to demonstrate the unique capabilities of micellar LC for chemical analysis which are especially advantages in solving bioanalytical problems.

这项工作的目的是探索极具前景的 胶束液相色谱的功能是一种强大的 化学分析和物理化学研究技术 生物医学研究。 该提案涉及系统性的 研究胶束 LC 的用途：表征 生物学上重要分子的理化性质和 环境污染物；能量学的研究和 溶质-胶束相互作用的机制及其对 胶束液相色谱的保留和选择性，以及 快速、灵敏的生化分析方法 化合物。 这三个项目共提出6个项目 目前引起人们广泛关注的主要领域 其内在的重要性，以及因为它们对广泛的影响 分离科学以外的一系列学科；从 药物化学到生物技术。 第一部分的目的是将胶束液相色谱应用于 有机化合物疏水特性的定量。 疏水性表征具有诊断和诊断作用 在药物设计等各个学科中的预测价值， 毒理学和环境监测。 胶束 LC 提供透明 与传统方法和 RPLC 相比的优势 用于估计疏水性的二元溶剂。 本研究的第二部分的目的是更好地定义 胶束液相色谱中保留和选择性的物理化学基础 通过精心设计的实验来突出 溶质-胶束相互作用的重要性。 最后，在第三部分，目的是展示独特的 胶束 LC 的化学分析能力包括 尤其是在解决生物分析问题方面的优势。