LYMPHOCYTE PHYSIOLOGY

淋巴细胞生理学

• 批准号: 3818226
• 负责人: T M CHUSED
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3818226
• 关键词: B lymphocyte; T lymphocyte; acidity /alkalinity; biological signal transduction; calcium metabolism; calcium transporting ATPase; flow cytometry; fluorescent dye /probe; granulocyte; lymphocyte; membrane permeability; membrane potentials; monocyte; phosphatidylinositols

The process of signal transduction across the lymphocyte plasma membrane is under investigation. Novel fluorescent probes of physiological parameters such as membrane potential, intracellular free ionized calcium, and intracellular pH, in conjunction with the high sensitivity and single cell resolution of flow cytometry, are being utilized. These studies have revealed extensive "feed- forward" and "feed-back" regulatory relationships between ion channel opening membrane potential, activity of the calcium pump, and rate of phosphatidyl inositol turnover. These mechanisms differ in the T, B and monocyte/granulocyte lineages.

跨淋巴细胞等离子体的信号转导过程 膜正在研究中。 新颖的荧光探针 生理参数，例如膜电位，细胞内 自由离子化钙和细胞内pH和 流式细胞术的高灵敏度和单细胞分辨率为 被利用。 这些研究揭示了广泛的“进食” 离子之间的前向”和“馈回”调节关系 通道打开膜电位，钙泵的活性， 和磷脂酰肌醇更新的速率。 这些机制 T，B和单核细胞/粒细胞谱系的不同。