LYMPHOCYTE PHYSIOLOGY

淋巴细胞生理学

• 批准号: 3809647
• 负责人: T M CHUSED
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3809647
• 关键词: B lymphocyte; T lymphocyte; acidity /alkalinity; biological signal transduction; calcium channel; calcium flux; calcium metabolism; calcium transporting ATPase; cell membrane; flow cytometry; fluorescent dye /probe; granulocyte; lymphocyte; membrane potentials; monocyte; phosphatidylinositols

The process of Signal transduction across the lymphocyte plasma membrane is under investigation. Novel fluorescent probes of physiologic parameters such as membrane potential, intracellular free ionized calcium, and intracellular pH, in conjunction with the high sensitivity and single cell resolution of flow cytometry, are being utilized. These studies have revealed extensive "feed-forward" and "feed-back" regulatory relationships between ion channel opening, membrane potential, activity of the calcium pump, and rate of phosphatidyl inositol turnover. These mechanisms differ in the T, B and monocyte/granulocyte lineages.

跨淋巴细胞质膜的信号转导过程是 正在调查。新型生理参数的荧光探针 例如膜电位，细胞内游离离子化钙和 细胞内pH，与高灵敏度和单细胞结合 流式细胞仪的分辨率正在利用。这些研究有 揭示了广泛的“进料”和“喂养”监管关系 在离子通道开口，膜电位，钙的活性之间 泵和磷脂酰肌醇更新的速率。这些机制不同 在T，B和单核细胞/粒细胞谱系中。