ENERGY TRANSDUCTION BY CYTOCHROME OXIDASE AND BACTERIORHODOPSIN

细胞色素氧化酶和细菌视紫红质的能量转换

• 批准号: 3779497
• 负责人: R W HENDLER
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3779497
• 关键词: acidity /alkalinity; bacteriorhodopsins; bioenergetics; cytochrome oxidase; evaporation; hydrogen transport; hydrogen transporting ATP synthase; liposomes; membrane reconstitution /synthesis; method development; optics; oxidative phosphorylation; phospholipids; photochemistry

Inverted E. coli vesicles and a variety of proteoliposome preparations were evaluated for reconstitution of bacteriorhodopsin (BR) to form a light-driven system to build and maintain high levels of delta muH+. The system chosen involves reversed phase evaporation of a suspension of purified phospholipids in an ether water mixture. Improved optics and electronics are under development to quantify the extents of Ph and electrical gradients that are formed. The system will be used to measure the quantum efficiency of proton pumping in low and high steady state levels of illumination and to probe the roles of deltapH and delta psi in the energetics of ATP synthesis and hydrolysis under catalysis of the membrane-bound ATP synthase, FoF1.

倒大肠囊泡和各种蛋白质脂体制剂 评估了对细菌淡淡蛋白酶（BR）的重建以形成A 轻驱动系统以构建和维持高水平的Delta Muh+。这 选择的系统涉及相反的相位蒸发 在醚水混合物中纯化的磷脂。改进的光学和 正在开发电子设备，以量化pH的范围和 形成的电梯度。该系统将用于测量 低稳态和高稳态质子泵送的量子效率 照明水平并探测Deltaph和Delta PSI的作用 在ATP合成和水解的能量学中 膜结合ATP合酶FOF1。