A synthetic yeast methanotroph for methane based sustainable biomanufacturing - SynYeast

用于基于甲烷的可持续生物制造的合成酵母甲烷氧化菌 - SynYeast

• 批准号: EP/Z000734/1
• 负责人: Ushasree Mrudulakumari Vasudevan
• 金额: $ 26.26万
• 依托单位: Aston University
• 依托单位国家: 英国
• 项目类别: Fellowship
• 财政年份: 2024
• 资助国家: 英国
• 起止时间: 2024 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FZ000734%2F1
• 关键词: synthetic; yeast; methanotroph; methane; based

Methane, the principal component of natural gas, is a powerful greenhouse gas driving climate change. Recent rises in global methane emission and the abundance of natural gas reserves have prompted interest in exploiting this one carbon compound as an industrial feedstock for bioproduction of liquid fuels and value-added chemicals. However, no direct methane to methanol conversion process have been commercialized at industrial scale. Bio-based manufacturing could provide novel solutions. The particulate methane monooxygenase (pMMO) found in methanotrophic bacteria is a selective catalyst for methane activation and conversion to methanol under mild conditions. Hence, much focus has been on heterologous expression of this enzyme in platform strains like E. coli to develop 'synthetic methanotrophs'. However, no scalable methane conversion has been demonstrated in E. coli due to the membrane-bound nature of this enzyme and the toxicity of methanol. Pichia pastoris is an efficient methylotroph with a native methanol assimilation pathway and an established host for membrane protein production. This project will express pMMO in the Pichia membrane to enable biological methane conversion. This strain will be further engineered to demonstrate the production of the high value product- 3-hydroxy propionic acid (a precursor of plastics) from methane. This engineered yeast will allow for rapid design-build-test cycles and is well-suited for industrial scale up. Building on a the Fellow's clear potential, with supervision and training at carefully chosen host/cohost, this innovative project will establish synthetic methanotrophy in a standard platform strain for production of valuable products, underpinning a sustainable circular economy.

甲烷是天然气的主要组成部分，是一种强大的温室气体驱动气候变化。最近的全球甲烷排放量和丰富的天然气储量促进了利用这种碳化合物作为工业原料的兴趣，用于生物生产液体燃料和增值化学品。但是，尚无直接甲烷到甲醇转化过程的工业规模商业化。基于生物的制造可以提供新颖的解决方案。在甲烷营养细菌中发现的颗粒甲烷单加氧酶（PMMO）是一种选择性催化剂，用于在轻度条件下甲烷激活和转化为甲醇。因此，在大肠杆菌等平台菌株中，这种酶的异源表达非常关注，以发展为“合成甲基营养”。但是，由于该酶的膜结合性质和甲醇的毒性，在大肠杆菌中尚未证明甲烷转化率。 Pichia Pastoris是一种有效的甲基营养物，具有天然甲醇同化途径，并且是膜蛋白产生的已建立宿主。该项目将在皮基亚膜中表达PMMO，以实现生物甲烷转化。该菌株将进一步设计，以证明甲烷的高价值产物-3-羟基丙酸（塑料的前体）的产生。该工程的酵母将允许快速设计建造测试周期，并且非常适合工业规模。该创新的项目以同胞的明确潜力，并在精心挑选的主机/同位主机上进行监督和培训，将在标准平台生产有价值产品的标准平台上建立合成的甲烷植物，并为可持续的循环经济提供支持。