LUBRICATION EFFECTS OF SALIVARY PELLICLES

唾液膜的润滑作用

• 批准号: 3775839
• 负责人: WILLIAM H DOUGLAS
• 金额: --
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3775839
• 关键词: adhesions; albumins; amphiphilicity; glycoproteins; human subject; mucins; nonblood rheology; pharmacokinetics; physical chemical interaction; protein purification; proteins; saliva; tooth enamel

The previous grant period established the structure-function relationships whereby salivary statherin and mucins take part in different hard tissue lubrication regimes. The renewal application proposes to define further and then enhance the physicochemical properties of lubricants at the enamel interface. The identification of new high lubricity amphipaths may lead to treatment modalities for the clinical manifestations of dental abrasion and attrition. The immediate objectives are the establishment of the complete paradigm of the boundary lubrication of enamel. We propose to study small model amphipathic compounds which will then assist us in the design of more complex macromolecules including statherin mutants developed in Subproject I.B. Different modes of delivery of lubricant molecules to enamel surface will be explored including: 1) model compounds in aqueous solutions; 2) insoluble films deposited on enamel from nonaqueous solvents; and 3) slow release delivery from dental material such as composite resins. These formats cover the spectrum of clinical possibilities and should lead to real systems capable of reducing interdental friction coefficient below 0.1 for prolonged periods of time. Investigations on natural salivas, purified mucins, mucin derivatives, and mucin/albumin complexes will also include water evaporative loss which is controlled by colligative properties and the rheological characteristics. These studies may elucidate the role of mucin solvation in the thick film component of thin film lubrication. Collectively, these studies will set new criteria for the performance of salivary substitutes which will include lubricity, water retention and flow characteristics, and in this way to enhance or improve upon the protective properties of saliva.

先前的资助期建立了结构-功能关系 唾液富酪蛋白和粘蛋白参与不同的硬组织 润滑制度。 续展申请建议进一步明确 然后增强釉质处润滑剂的理化性能 界面。 新的高润滑性两亲物的鉴定可能会导致 针对牙齿磨损临床表现的治疗方法 磨损。 近期目标是建立完整的 牙釉质边界润滑的范例。 我们建议学习小 两亲化合物模型，这将有助于我们设计更多 子项目中开发的复杂大分子，包括富酪蛋白突变体 I.B. 润滑剂分子向牙釉质表面输送的不同方式 将探索包括：1）水溶液中的模型化合物； 2） 非水溶剂沉积在牙釉质上的不溶性薄膜； 3）慢 从复合树脂等牙科材料中释放释放。 这些 格式涵盖了临床可能性的范围，并且应该导致 能够将齿间摩擦系数降低到 0.1 以下的真实系统 长时间。 对纯化的天然唾液的研究 粘蛋白、粘蛋白衍生物和粘蛋白/白蛋白复合物还包括 水蒸发损失，由依数性质控制， 流变特性。 这些研究可能会阐明 厚膜中的粘蛋白溶剂化成分起到薄膜润滑的作用。 总的来说，这些研究将为性能设定新的标准 唾液替代品，包括润滑性、保水性和流动性 特性，并以此方式增强或改进保护性 唾液的特性。