LUBRICATION EFFECTS OF SALIVARY PELLICLES

唾液膜的润滑作用

• 批准号: 3854356
• 负责人: WILLIAM H DOUGLAS
• 金额: --
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3854356
• 关键词: biological information processing; biomechanics; bite strength; bruxism; chemical structure function; fluidity; glycoproteins; glycosylation; human subject; intermolecular interaction; molecular film; neural information processing; oral bacteria; pellicle; saliva

The objectives of this application are to quantify the lubrication effects of human salivary macromolecules at the enamel interface and to identify the biological advantages of such lubrication. The proposal will seek to test materials of supersalivary performance as part of the overall goal of the parent RCOB proposal to enhance or improve upon nature. The specific aims are: (1) The development of an enamel substrate to provide baseline studies from which all other effects can be measured. The aim here is also to identify the biological consequences of the absence of a salivary pellicle in respect to such tribological properties as friction, wear and wear mechanism. (2) The second aim will be to construct a clear picture of the tribological properties of selected salivary macromolecules, previously demonstrated to exhibit lubrication activity. This will involve studies on human salivary glycoproteins including the proline rich glycoprotein and high and low molecular weight salivary mucins. In addition, we will chemically modify those molecules to ascertain the structural domain involved in lubricity. Such derivatives will be deglycosylated, deacylated or reduced and allcylated. The potential enhancement of lubrication by heterotypic complexing between human serum albumin and individual glycoproteins (plus their derivatives) will be explored. Finally, bi- and tri-composite salivary molecules (neoglycoproteins developed in subproject I.A.) will be tested. The methodology to achieve these objectives is based on servohydraulics configured as an artificial oral environment. Stimulated studies on natural teeth will be performed under complete environmental control. Frictional forces and wear mechanisms will be determined under different conditions. Special emphasis is laid on the integration of the proposal with the biochemical study of the overall RCOB proposal at SUNY at Buffalo. It is anticipated that such an integrated approach to the basic study of saliva would indicate synthetic routes to salivary materials of enhanced and prolonged lubrication effects, an essential requirement for the clinical management of salivary dysfunction and occlusal abrasion.

该应用的目的是量化润滑 人类唾液大分子对牙釉质的影响 界面并确定此类的生物学优势 润滑。 该提案将寻求测试材料 超唾液性能作为总体目标的一部分 家长 RCOB 提案旨在增强或改进性质。 这 具体目标是： (1) 釉质基材的开发提供基线 可以衡量所有其他影响的研究。 目的 这里还要确定缺席的生物学后果 唾液膜的摩擦学特性如下 摩擦、磨损和磨损机理。 (2) 第二个目标是构建一个清晰的图景 选定的唾液大分子的摩擦学特性， 先前已证明具有润滑活性。 这将 涉及人类唾液糖蛋白的研究，包括 富含脯氨酸的糖蛋白和高、低分子量 唾液粘蛋白​​。 此外，我们还会对这些进行化学修饰 分子以确定参与润滑性的结构域。 此类衍生物将被去糖基化、脱酰基化或还原 和烷基化。 潜在的润滑增强作用 人血清白蛋白与人血清白蛋白之间的异型络合 将探索单个糖蛋白（及其衍生物）。 最后，双复合和三复合唾液分子 （子项目 I.A. 中开发的新糖蛋白）将进行测试。 实现这些目标的方法基于 配置为人工口腔环境的伺服液压系统。 对天然牙齿的刺激研究将在 完善的环境控制。 摩擦力和磨损 机制将根据不同情况确定。 特别强调该提案与 纽约州立大学 (SUNY) 总体 RCOB 提案的生化研究 水牛。 预计这种综合方法将 唾液的基础研究将表明唾液的合成途径 增强和延长润滑效果的材料， 唾液临床管理的基本要求 功能障碍和咬合磨损。