HPLC ANALYSIS OF PURINE NUCLEOSIDES IN CREVICULAR FLUID

沟液中嘌呤核苷的高效液相色谱分析

• 批准号: 3424841
• 负责人: EDWARD F ROSSOMANDO
• 金额: $ 2.28万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-01-01 至 1986-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3424841
• 关键词: blood chemistry; dental disorder diagnosis; gingival sulcus; high performance liquid chromatography; nucleotides; oral bacteria; periodontium disorder; phosphorylation; purine nucleosides; saliva

The long term objective of this work is to analyze the fluid from the gingival crevice of humans in an attempt to discover a component which can be used to establish criteria for the health status of the periodontium. In this proposal, initial studies on the application of high performance liquid chromatography (hplc) technology to this problem are described. In these studies crevicular fluid will be collected and analyzed by reverse phase and ion exchange hplc for purine nucleobases, nucleosides and nucleotides using techniques previously worked out in this laboratory. Based on preliminary studies, such compounds should present and should show that a correlation exists between pocket depth and the level of phosphorylated nucleosides. The specific aims of the proposal include 1) the continue development of the hplc technology including studies on both stationary and mobile phases, 2) the analysis of samples previously collected to assess reproductability of technique and 3) to analyze samples to explore the problem from saliva, blood and bacteria. It is the intent of this study to apply hplc technology to the problem of periodontal disease by providing clinically-useful guidelines to assess the status of the periodontium.

这项工作的长期目标是分析来自 人类的牙龈缝隙，试图发现一种可以 用于建立牙周组织健康状况的标准。 在本提案中，初步研究了高性能应用 液相色谱（hplc）技术对这一问题进行了阐述。 在 这些研究将收集裂隙液并通过反向分析进行分析 相和离子交换 HPLC，用于检测嘌呤核碱基、核苷和 使用该实验室先前制定的技术来检测核苷酸。 根据初步研究，此类化合物应存在并应显示 口袋深度和水平之间存在相关性 磷酸化核苷。 该提案的具体目标包括 1) HPLC 技术的持续发展，包括对两者的研究 固定相和流动相，2) 之前样品的分析 收集用于评估技术的再现性和 3) 分析样品 从唾液、血液和细菌来探究问题。 本研究的目的是将高效液相色谱技术应用于解决 通过提供临床有用的指南来评估牙周病 牙周组织的状态。

项目成果

HLAMP--a conjugate of hippuryllysine and AMP which contains a phosphoamide bond--stimulates chemotaxis in Dictyostelium discoideum.

HLAMP（马尿酰赖氨酸和 AMP 的结合物，含有磷酰胺键）可刺激盘基网柄菌的趋化性。

• 发表时间: 1987
• 作者: Rossomando, E F;Hadjimichael, J;Varnum;Soll, D R
• 通讯作者: Soll, D R