BIOCHEMICAL PROFILE FOR GINGIVAL CREVICULAR FLUID

龈沟液的生化特征

• 批准号: 3221034
• 负责人: IRA B LAMSTER
• 金额: $ 11.35万
• 依托单位: FAIRLEIGH DICKINSON UNIVERSITY TEANECK
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-01 至 1988-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3221034
• 关键词: acute phase protein; arylsulfatases; beta glucuronidase; biochemistry; body fluids; collagenase; dental alveolus; dental disorder diagnosis; erythema; gingival sulcus; human tissue; immunodiffusion; inflammation; oral bacteria; peptidases; periodontium disorder; spectrometry; trypsin

Traditional measures of periodontal disease activity (erythema, bleeding on probing, suppuration and pocket depth), while reflecting some degree of pathology in the tissues, are not reflective of disease activity as defined by loss of periodontal attachment or loss of alveolar bone. Consequently, specific and sensitive measures of periodontal disease activity are required. While considerable effort has been devoted to defining the chemistry of gingival crevicular fluid (GCF), to date GCF analysis has not aided the clinician in managing the periodontal patient. A careful review of this literature, however, indicates that problems exist in regard to the methodology of previous studies. Work in our laboratory has suggested a technique for GCF chemistry that involves sampling of individual crevicular sites for 30 seconds using precut filter paper strips. The volume of fluid is quantitated using a calibrated GCF meter, and then the material on the strip is eluted into 300-400 Mul of diluent. Aliquots of the diluent are then used to evaluate multiple constituents in the sample. The advantages of this technique are: 1. The sample of GCF represents the fluid present in the crevice. Collecting a standardized 1, 2 or 5 Mul from any one site causes contamination of GCF with serum from disrupted capillary beds. 2. The volumetric requirements of the analytical assays are met, and 3. Multiple components in the GCF can be analyzed from the same sample. Our studies with this system suggests that GCF can be a sensitive measure of pathology in the periodontium. This proposal intends to utilize a GCF chemical profile to evaluate periodontal disease activity (loss of periodontal attachment, occurrence of a periodontal abscess or loss of osseous support upon radiographic evaluation). Specifically, this project will analyze GCF samples from the individual crevicular sites for the: 1. Activity of the enzymes beta-glucuronidase, arylsulfatase, collagenase (active and total) and prolidase using spectrophotometric techniques, and 2. Use immunodiffusion techniques to analyze for the antiproteases alpha-1-antitrypsin and alpha-2-macroglobulin, and the systemic marker of inflammation C-reactive protein. By evaluating factors intimately involved in the inflammatory reaction, and the breakdown of nonmineralized and mineralized connective tissue, this research project will determine if changes in the pattern of the GCF profile precede, coincide with or follow the onset of periodontal disease activity in man.

牙周病活动性的传统测量（红斑、出血） 探查、化脓和袋深度），同时反映一定程度的 组织中的病理学，并不反映所定义的疾病活动 由于牙周附着丧失或牙槽骨丧失。 最后， 牙周病活动的特异性和敏感测量是 必需的。 尽管我们付出了相当大的努力来定义 龈沟液 (GCF) 的化学成分，迄今为止 GCF 分析还没有 帮助临床医生管理牙周患者。 仔细审查 然而，该文献表明，在 以前的研究方法。 我们实验室的工作建议 GCF 化学技术，涉及对单个缝隙进行采样 使用预切滤纸条静置 30 秒。 液体体积 使用校准的 GCF 计进行定量，然后将材料放在 将条带洗脱到300-400μl的稀释剂中。 稀释剂的等分试样是 然后用于评估样品中的多种成分。 优点 该技术的主要内容是： 1. GCF 样本代表缝隙中存在的流体。 从任何一个地点收集标准化 1、2 或 5 Mul 会导致 GCF 被毛细血管床破裂的血清污染。 2. 满足分析测定的体积要求，并且 3. 可以从同一样品中分析GCF中的多种成分。 我们对该系统的研究表明 GCF 可以是一种敏感的衡量标准 牙周组织的病理学。 该提案旨在利用 GCF 用于评估牙周病活动性的化学特征（牙周病活性丧失） 牙周附着、发生牙周脓肿或牙周脱落 射线照相评估的骨支持）。 具体来说，这个项目 将分析来自各个缝隙部位的 GCF 样本： 1. β-葡萄糖醛酸酶、芳基硫酸酯酶、胶原酶的活性 （活性和总）和脯氨酸酶使用分光光度技术，和 2. 使用免疫扩散技术分析抗蛋白酶 α-1-抗胰蛋白酶和α-2-巨球蛋白，以及全身性标志物 炎症C反应蛋白。 通过评估与炎症反应密切相关的因素，以及 非矿化和矿化结缔组织的分解，这 研究项目将确定 GCF 模式是否会发生变化 轮廓在牙周病发作之前、同时或之后 人体内的活动。