FOURIER TRANSFORM INFRARED/RAMAN SPECTROMETER

傅里叶变换红外/拉曼光谱仪

基本信息

批准号：
2284216
负责人：
MARK S BRAIMAN
金额：
$ 15.15万
依托单位：
UNIVERSITY OF VIRGINIA
依托单位国家：
美国
项目类别：
财政年份：
1994
资助国家：
美国
起止时间：
1994-07-27 至 1995-07-26
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2284216
关键词：
biomedical equipment purchase digital imaging infrared spectrometry interferometry lasers membrane structure structural biology time resolved data

项目摘要

A state=of-the-art- Fourier-transform (FT) infrared/Raman spectrometer will be used o perform vibrational spectroscopic studies of biomolecules. The instrument selected, a Digilab FTS60A, is capable of step-scanning interferograms obtained either from broadband IR light that is attenuated at selected wavelengths by a thin biological sample, or from near-IR light from an intense 1064-nm laser that is scattered and shifted to a variety of longer wavelengths via the Raman effect. The step-scanning capability of the instrument, along with its fast (400 KHz) digitizer with 19-bit dynamic range, will make it possible to obtain broadband time-resolved IR absorption and Raman scattering measurements with time resolution as fast as 5-mus. Furthermore, using a high- repetition-rate Q-switched Nd+-YAG laser should permit time resolution for Raman experiments down into the 10 nsec range. The beamsplitter and detector combination to be obtained along with spectrometer will allow complete spectral coverage from 10,000-100 cm in the infrared, and of measurements of Stokes-shifted Raman bands from 4,000-75cm. A microscope accessory for the FTS60A will allow sensitive IR absorbance difference measurements to be made on tiny quantities of sample (1 ng). The NIH-supported core group is planning to use the spectrometer principally to examine the structure and dynamics of biomembrane components, including simple lipid bilayer systems, supported planar bilayers with small peptides bound to them, and moderately large membrane proteins reconstituted into lipid vesicles. The proposed core users have all independently published NIH-supported papers demonstrating a capability and interest in using FT-IR and/or Raman spectroscopy to answer structural questions about membrane components involved in a variety of biomedically-important processes such as visual transduction, transmembrane chloride transport, and influenza virus insertion into cell membranes.

一个状态= - 傅里叶转变（FT）红外/拉曼光谱仪将使用o进行生物分子的振动光谱研究。选定的仪器是Digilab FTS60A，能够踩踏从宽带红外光中获得的干涉图在选定的波长下，由薄的生物样品或接近IR 来自散射并转移到A的强烈1064 nm激光器的光通过拉曼效应多种较长的波长。乐器的阶梯扫描能力以及其快速（400 KHz）具有19位动态范围的数字化器将使获得宽带时间分辨的IR吸收和拉曼散射测量值时间分辨率与5-MU一样快。此外，使用高重复率Q开关ND+-YAG激光器应允许时间分辨率对于拉曼实验至10个NSEC范围。将获得的Beamsplitter和检测器组合以及光谱仪将允许10,000-100厘米的完整光谱覆盖红外线，以及来自stokes偏移的拉曼乐队的测量 4,000-75厘米。 FTS60A的显微镜配件将允许敏感 IR吸光度差异测量值很少样品（1 ng）。 NIH支持的核心组计划使用光谱仪主要检查生物膜的结构和动力学组件，包括简单的脂质双层系统，支持平面与它们结合的小肽的双层和中等大膜蛋白质重构为脂质囊泡。拟议的核心用户有所有独立发表的NIH支持的论文都证明了对使用FT-IR和/或拉曼光谱的能力和兴趣回答有关涉及膜成分的结构性问题各种生物医学重要过程，例如视觉转导，跨膜氯化物转运和流感病毒插入细胞膜。