TIME RESOLVED VIBRATIONAL SPECTROSCOPY OF HALORHODOPSIN

盐视紫红质的时间分辨振动光谱

• 批准号: 2392162
• 负责人: MARK S BRAIMAN
• 金额: $ 10.74万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 1999-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2392162
• 关键词: Raman spectrometry; chloride channels; gene mutation; infrared spectrometry; interferometry; protein structure function; rhodopsin; site directed mutagenesis; time resolved data

DESCRIPTION: Studying the photocycle of halo rhodopsin, a chloride pump, with FTIR as well as FT visible and FT Raman. Renewal request of a previous 3 year award, now back to BBCA as an amended proposal. Specific aims are to resolve the photocycle of hR. Examine whether or not the Schiff base frequency is halide dependent and whether or not bands from the arginine side groups are also halide dependent. Then, try to identify which args are those showing up in the IR difference spectrum. One last goal is to obtain IR difference spectra in response to, with and without halide salts in solution, using an ATR cell and flowing the ions. A new faster (5 microsecond) FT-IR spectrometer has just been installed. This will provide about three orders of magnitude better resolution.

描述：研究光紫红质的光循环，氯化物泵， 与FTIR以及FT可见和FT拉曼。 续签请求 前三年奖，现在回到BBCA作为修订的提案。 具体目的是解决HR的光循环。 检查是否或 Schiff的基本频率不是卤化物依赖性的，是否 精氨酸侧基的带也依赖于卤化物。 然后， 尝试确定哪些ARG是在IR差异中显示的ARG 光谱。 最后一个目标是获得响应IR差异光谱 在溶液中使用和不带有卤化物盐，使用ATR电池和 流动。 新的更快（5微秒）的FT-IR光谱仪具有 刚刚安装。 这将提供大约三个数量级 更好的分辨率。