PHYSIOLOGY OF MUSCARINIC SYNAPSES IN SYMPATHETIC GANGLIA

交感神经节毒蕈碱突触的生理学

• 批准号: 3401845
• 负责人: JOHN P HORN
• 金额: $ 9.96万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1990-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3401845
• 关键词: Anura; acetylcholine; action potentials; adenosine triphosphate; adrenergic receptor; aorta coarctation; artery; binding proteins; calcium transporting ATPase; denervation; electrical conductance; electrodes; electrophysiology; epinephrine; fluorescent dye /probe; fresh water environment; goldfish; guanosine triphosphate; innervation; membrane potentials; monoclonal antibody; muscarinic receptor; neural transmission; neuroanatomy; neuroendocrine system; neuromuscular transmission; neuropeptide Y; neuropeptide receptor; neuropeptides; neurotransmitters; parasympathetic nervous system; phentolamine; prazosin; slow potential; stimulus /response; stimulus interval; sympathetic ganglion; synapses; tissue /cell culture; vascular smooth muscle nervous control; vasoconstriction

Sympathetic ganglia convey signals that mediate adaptive responses to stress. The long-term goals of this project are to understand two fundamental aspects of normal sympathetic physiology. The first goal is to identify functional subsystems of sympathetic neurons that innervate selectively different classes of peripheral targets (eg. blood vessels vs. glands) and the second goal is to determine the integrative role of slow synaptic transmission in sympathetic ganglia. The underlying hypotheses are that functional subclasses of sympathetic neurons release different combinations of co-transmitters to control their respective targets and that slow synaptic potentials in ganglia serve to modulate the firing of repetitive action potentials thereby providing a mechanism for regulating the release of individual co-transmitters from a given class of ganglion cells. The most important aspect of this project is to provide a model for understanding the physiological significance of muscarinic and peptidergic synapses in autonomic ganglia. In addition, this research will develop an in vitro system for identifying cellular and molecular mechanisms that regulate phenotypic properties of functionally distinct sympathetic neurons. Using bullfrogs, a combination of electrophysiological and anatomical methods will be used to address four specific aims: 1) Test the hypothesis that sympathetic C cells are vasomotor neurons that utilize epinephrine (EPI), neuropeptide Y (NPY) and adenosine triphosphate (ATP) as co-transmitters. 2) Determine how muscarinic and peptidergic synaptic potentials in ganglia and the repetitive firing that they engender, each regulate the release of EPI, NPY and ATP from C neurons onto arteries. 3) Develop a tissue culture system in which a vasomotor C cells can be identified and the use patch clamp methods to characterize the membrane currents that produce and modulate the repetitive firing of action potentials. 4) Test the hypothesis that fast B and/or slow B but not C-type sympathetic neurons innervate cutaneous mucous glands and granular glands. Then find suitable anatomical markers that can be used to trace the peripheral projections of B-type neurons and to identify dissociated B neurons in tissue culture

交感神经节传递调节适应性的信号 对压力的反应。 该项目的长期目标是 了解正常交感神经的两个基本方面 生理。 第一个目标是确定功能子系统 选择性地支配不同类别的交感神经元 外周目标（例如血管与腺体）和第二个 目标是确定慢突触的整合作用 交感神经节中的传递。 基本假设 交感神经元的功能亚类释放 共同发射器的不同组合来控制它们 各自的目标并减缓神经节的突触电位 用于调节重复动作电位的激发 从而提供调节释放的机制 来自给定类别的神经节细胞的个体协同递质。 这个项目最重要的方面是提供一个模型 为了理解毒蕈碱和毒蕈碱的生理意义 自主神经节中的肽能突触。 此外，这 研究将开发一种体外系统来识别细胞 以及调节表型特性的分子机制 功能不同的交感神经元。 使用牛蛙，结合电生理学和 解剖学方法将用于实现四个具体目标： 1）检验交感C细胞具有血管舒缩作用的假设 利用肾上腺素 (EPI)、神经肽 Y (NPY) 和 三磷酸腺苷（ATP）作为共递质。 2) 确定毒蕈碱和肽能突触电位 在神经节和它们产生的重复放电中，每个 调节 C 神经元释放 EPI、NPY 和 ATP 动脉。 3) 开发一种组织培养系统，其中血管舒缩 C 细胞 可以识别并使用膜片钳方法 表征产生和调节的膜电流 动作电位的重复放电。 4) 检验快速 B 和/或慢速 B 但不是 C 型的假设 交感神经元支配皮肤粘液腺 颗粒状腺体。 然后找到合适的解剖标记 用于追踪 B 型神经元的外周投射 鉴定组织培养物中分离的 B 神经元