MULTIUSER DESORPTION MASS SPECTROMETER

多用户解吸质谱仪

• 批准号: 2283822
• 负责人: Catherine C Fenselau
• 金额: $ 10万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-30 至 1995-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2283822
• 关键词: biomedical equipment purchase; biomedical equipment resource; mass spectrometry

A budget is requested for acquisition of a benchtop instrument for matrix assisted laser desorption mass spectrometry to provide rapid determinations of molecular weights in conjunction with studies of protein processing, modification and structure-function relations. The use of membranes will be developed as surfaces for matrix assisted laser desorption, including desorption of the products of protease digestion and chemical reactions carried out directly on the membrane. Collaborative studies of modified oligonucleotides, of polysaccharides and of noncovalent protein complexes will include methods development in those areas. It is intended that this installation will operate part of the time in a walk-up, multi-user mode, allowing molecular biologists and biochemists to make their own molecular weight determinations. Training and proof of method will precede most of these applications.

要求收购矩阵台式工具的预算 辅助激光解吸质谱法以提供快速 与蛋白质的研究一起测定分子量 处理，修改和结构功能关系。使用 膜将作为矩阵辅助激光的表面开发 解吸，包括蛋白酶消化产物的解吸和 化学反应直接在膜上进行。协作 修改的寡核苷酸，多糖和的研究 非共价蛋白质复合物将包括其中的方法开发 区域。 目的是，此安装将在一部分时间内运行 步行，多用户模式，允许分子生物学家和生物化学家 做出自己的分子量测定。培训和证明 方法将在大多数这些应用程序之前。