THE NEURAL CONTROL OF A LACRIMAL GLAND

泪腺的神经控制

• 批准号: 3399400
• 负责人: BENJAMIN WALCOTT
• 金额: $ 8万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-04-01 至 1986-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3399400
• 关键词: B lymphocyte; antibody formation; electron microscopy; histochemistry /cytochemistry; immunochemistry; immunoglobulin A; lacrimal apparatus; microscopy; neural information processing; norepinephrine; secretion; vasoactive intestinal peptide

The Harderian gland is a major lacrimal gland found in all animals with a nictitating membrane. The gland contains two major cell populations, a tubular secretory epithelium which secretes a serous fluid and lymphoid tissue consisting primarily of B-cells which secrete IgA. The gland is densely innervated and our preliminary data show that noradrenalin (NA) containing fibers are present as well as substance P-like, VIP-like and eukephalin-like immunoreactivity. Further these anatomical studies show that NA fibers are morphologically closer to the B-cells ihan to other cell types. Since there is some evidence for neuronal modulation of antibody production in spleen we will test this hypothesis in Harderian gland where antibody production in the tears can be measured. We will use immunocytochemical techniques at the light and electron microscope levels to determine the details of the innervation pattern. We will examine the pattern in chicks at different ages as the full complement of B-cells is not found until about 1 month before hatching. We will collect tears of normal and stimulated birds to see if antibody production is regulated as is secretion of the serous fluid.

哈德氏腺是一种主要的泪腺，存在于所有具有泪腺的动物中。 瞬膜。 该腺体包含两个主要细胞群，一个 管状分泌上皮，分泌浆液和淋巴液 主要由分泌 IgA 的 B 细胞组成的组织。 腺体是 神经支配密集，我们的初步数据表明去甲肾上腺素 (NA) 含有纤维以及 P 类物质、VIP 类物质和 尤克啡肽样免疫反应性。 这些解剖学研究进一步表明 NA 纤维在形态上更接近于 B 细胞，更接近于其他细胞 类型。 由于有一些证据表明抗体的神经元调节 我们将在哈德氏腺中检验这个假设，其中 可以测量眼泪中抗体的产生。 我们将使用 光学和电子显微镜水平的免疫细胞化学技术 以确定神经支配模式的细节。 我们将检查 不同年龄雏鸡的模式，因为 B 细胞的完整补充是 直到孵化前约1个月才被发现。 我们将收集泪水 正常和受刺激的鸟类，看看抗体的产生是否受到调节 是浆液的分泌。

项目成果

Neuropeptides and the innervation of the avian lacrimal gland.

神经肽和禽泪腺的神经支配。

• 发表时间: 1989-07
• 影响因子: 4.4
• 作者: Walcott, B;Sibony, P A;Keyser, K T
• 通讯作者: Keyser, K T

Iron-containing cells in the honey-bee (Apis mellifera). I. Adult morphology and physiology.

蜜蜂（Apis mellifera）中的含铁细胞。

• 发表时间: 1986-11
• 作者: Kuterbach, D A;Walcott, B
• 通讯作者: Walcott, B