FLUORESCENCE STUDIES OF BIOPHYSICAL PROPERTIES OF POLYUNSATURATED PHOSPHOLIPIDS

多不饱和磷脂生物物理性质的荧光研究

• 批准号: 5200231
• 负责人: B LITMAN
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5200231
• 关键词: biophysics; drug interactions; drug tolerance; ethanol; fatty acylation; fluorescent dye /probe; lipid bilayer membrane; long chain fatty acid; membrane activity; membrane lipids; membrane structure; phase change; phospholipids; unsaturated fatty acids

Chronic alcohol exposure is known to cause resistance to ethanol-induced membrane disordering measured in vitro, usually referred to as membrane tolerance. Initial studies on the phospholipid acyl chain dependence of the effects of alcohol demonstrate that polyunsaturated acyl chains potentiate the effect of alcohol. This finding, coupled with the observation made in this Laboratory that alcohol depletes the long-chain polyunsaturated fatty acids, such as 20:4n6 and 22:6n3, may provide an explanation for the development of the tolerance effect. A novel model for phospholipid acyl chain packing, developed in this Laboratory, includes the formation of lateral domains, which is driven by the strong interactions of the saturated sn-1 chains relative to the polyunaturated sn-2 chains. This model is supported by our findings that mixtures of disaturated PCs and a dipolyunsaturated (di22:6n3) PC show lateral phase separation in the gel phase. Phase transition studies of a variety of mixed-chain phosphatidylethanolamines (PE) demonstrate that this class of phospholipids can undergo a liquid crystalline to hexagonal II phase transition in the physiological temperature range and may in this way provide unique structural features to the membranes in which they reside.

已知慢性酒精暴露会引起对乙醇诱导的抗性 体外测量的膜无序，通常称为膜 宽容。 关于磷脂酰基链依赖性的初步研究 酒精的作用表明多不饱和酰基链 增强酒精的影响。 这个发现，加上 在这个实验室中观察到酒精会耗尽长链 多不饱和脂肪酸，例如20：4n6和22：6n3，可能会提供 解释耐受效应的发展。 在此开发的一种新型磷脂酰基链链堆积模型 实验室包括横向域的形成，该域是驱动的 通过相对于饱和的SN-1链的强相互作用 多不饱和SN-2链。 我们的发现支持了这个模型 有缺陷的PC和二元饱和的混合物（DI22：6N3）PC 在凝胶相中显示侧相分离。 相变研究 多种混合链磷脂酰乙醇胺（PE）证明了 这类磷脂可以经历液晶到 在生理温度范围内的六边形II相变和 可以通过这种方式为膜提供独特的结构特征 他们居住。