POST-IMPLANTATION MODIFICATION OF THE SPIRAL ARTERIES

螺旋动脉的植入后改造

• 批准号: 3325109
• 负责人: BARRY F KING
• 金额: $ 15.16万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-03-01 至 1992-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3325109
• 关键词: Macaca mulatta; collagen; connective tissue; histochemistry /cytochemistry; immunochemistry; lysosomes; nonsurgical revascularization; pregnancy circulation; pregnancy toxemia /hypertension; scanning electron microscopy; trophoblast; vascular endothelium; vascular smooth muscle

The blood supply to the primate placenta is established by the conversion of the muscular spiral arteries into dilated funnel- shaped uteroplacental arteries whose walls are composed mainly of trophoblast cells and connective tissue. These changes are thought to accommodate the greater volume of blood flow required by the developing conceptus, and to modify the normal maternal control mechanisms of these vessels. It has been observed that pregnancies complicated by preeclampsia alone, or preeclampsia with essential hypertension, structural modification of the arterial walls is less extensive and this apparently compromises uteroplacental perfusion. The key element in the conversion of the arteries is the migration of endovascular cells, thought to be trophoblast, into the spiral arteries. The endothelium is apparently removed and this is followed by the destruction of the muscular arterial wall and its replacement by trophoblast and connective tissue. There are many gaps in our understanding of the development of the uteroplacental arteries in primates especially the cellular mechanisms by which the invasion, destruction and replacement are carried out. The long- term objective of this proposal is the characterize the process of invasion and modification of the spiral arteries in the rhesus monkey, a species with many placental anatomical similarities to the human. Specific aims are 1) to define ultrastructurally and immunocytochemically the sequence of cellular interactions between endovascular trophoblast and endothelial cells during the initial invasion of the spiral arteries; 2) to determine the cellular mechanisms by which the endovascular trophoblast removes the maternal endothelium, penetrates the walls of the arteries and destroys the smooth muscle of the tunica media. At the present time, there is little information available on how this is accomplished in primates. Careful ultrastructural descriptions will be coupled with cytochemical studies to evaluate the role of lysosomal enzymes in this process, a role suggested by studies in other species; and 3) to determine, using immunocytochemical methods, the changes in the composition of the connective tissue components of the arterial wall during the conversion of the spiral arteries to the uteroplacental arteries.

灵长类动物胎盘的血液供应是由 将肌性螺旋动脉转变为扩张的漏斗状动脉 成形的子宫胎盘动脉，其壁主要由 滋养层细胞和结缔组织。 这些变化是 被认为可以容纳更大的血流量 发育中的概念所需要的，并修改正常的 这些血管的母体控制机制。 它一直 观察到妊娠仅因先兆子痫而并发，或 先兆子痫伴原发性高血压，结构改变 动脉壁的范围较小，这显然 损害子宫胎盘灌注。 中的关键要素是 动脉的转换是血管内细胞的迁移， 被认为是滋养层，进入螺旋动脉。 这 内皮明显被去除，随后是 肌性动脉壁的破坏及其替代 滋养层和结缔组织。 我们的差距还有很多 了解子宫胎盘动脉的发育 在灵长类动物中，尤其是细胞机制 进行入侵、破坏和替换。 长- 该提案的长期目标是描述以下过程的特征： 恒河猴螺旋动脉的侵袭和改变 猴子，一种胎盘解剖学与猴子有许多相似之处的物种 人类。 具体目标是 1) 定义超微结构和 免疫细胞化学细胞相互作用的顺序 血管内滋养层和内皮细胞之间 最初侵入螺旋动脉； 2）确定细胞 血管内滋养层去除血管内细胞的机制 母体内皮细胞，穿透动脉壁 破坏中膜的平滑肌。 目前 时间，关于这是如何发生的信息很少 在灵长类动物身上取得了成就。 仔细的超微结构描述 将与细胞化学研究相结合来评估 溶酶体酶在此过程中发挥作用，研究表明 其他物种； 3) 使用免疫细胞化学来确定 方法，结缔组织成分的变化 螺旋转换过程中动脉壁的组成部分 动脉至子宫胎盘动脉。