CYTOLOGICAL STUDIES OF PRIMATE VAGINAL EPITHELIUM

灵长类阴道上皮的细胞学研究

基本信息

批准号：
3312418
负责人：
BARRY F KING
金额：
$ 8.8万
依托单位：
UNIVERSITY OF CALIFORNIA AT DAVIS
依托单位国家：
美国
项目类别：
财政年份：
1980
资助国家：
美国
起止时间：
1980-04-01 至 1986-03-31
项目状态：
已结题

项目摘要

This proposal will address two major questions critical to the understanding of the structure and physiology of vaginal epithelium. These are: 1) What is the nature of the permeability barrier in vaginal epithelium?, and 2) What are the properties of the vaginal cell surface that influence the adherence of bacteria to the cells? To test the hypothesis that intercellular substances may form part of the permeability barrier, histochemical and cytochemical studies will be conducted to demonstrate the nature and localization of lipids, glycoconjugates, and lysosomal enzymes in human and monkey vaginal epithelium. The results will be compared to localizations found in other keratinizing and non-keratinizing epithelia. Permeability barriers in intact epithelia will be further examined by injecting electro-dense tracer molecules (lanthanum and peroxidase) into the lamina propria of monkey vagina and following by electron microscopy the movement of the tracers into the epithelium. Similar experiments will be carried out with topically applied tracers to examine permeability barriers of the surface cells. Human vaginal epithelium will be grown to confluence in a cell culture system and the morphological differentiation, cytochemistry and permeability of this epithelium will be examined by similar techniques and compared to non-cultured epithelium. To study bacterial adherence to the cultured epithelium, optimal concentrations of Lactobacillus will be added to the cultured epithelium and their adherence quantitated. The effects of pH, temperature, divalentcations, and modification of cell surface carbohydrates on adherence will be measured.

该提案将解决对经济发展至关重要的两个主要问题了解阴道上皮的结构和生理学。这些是： 1) 阴道渗透性屏障的本质是什么上皮？和 2) 阴道细胞表面的特性是什么影响细菌与细胞粘附的因素？测试假设细胞间质可能形成渗透性的一部分将进行屏障、组织化学和细胞化学研究证明脂质、糖复合物的性质和定位人和猴阴道上皮中的溶酶体酶。结果将与其他角化和中发现的定位进行比较非角化上皮。完整上皮细胞的渗透性屏障将通过注入电致密示踪分子（镧和过氧化物酶）进入猴阴道固有层，然后电子显微镜观察示踪剂进入上皮的运动。将使用局部应用的示踪剂进行类似的实验检查表面细胞的渗透屏障。人类阴道上皮细胞将在细胞培养系统中生长至汇合，并且形态分化、细胞化学和通透性上皮细胞将通过类似的技术进行检查并与非培养上皮。研究细菌对培养物的粘附上皮细胞中，将添加最佳浓度的乳酸菌培养的上皮及其粘附定量。 pH值的影响，温度、二价阳离子和细胞表面的修饰将测量碳水化合物的依从性。