B CELL STIMULATORY FACTOR-1 (BSF-1)/INTERLEUKIN-4

B 细胞刺激因子-1 (BSF-1)/白细胞介素-4

• 批准号: 3809675
• 负责人: W E PAUL
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3809675
• 关键词: B lymphocyte; Nippostrongylus; T lymphocyte; antibody formation; antibody receptor; calcium; gene rearrangement; immunoglobulin E; immunoglobulin G; immunoglobulin genes; immunoregulation; interleukin 2; interleukin 4; ionophores; laboratory mouse; leukocyte activation /transformation; lymph nodes; lymphokines; mast cell; tissue /cell culture

Interleukin 4 (IL-4) is potent lymphokine that regulates a wide variety of functions of lymphoid and other hematopoietic cells. An assay to measure IL-4 production by single cells indicates has been developed. It shows that the frequency of T cells capable of producing Il-4 in lymph node cell suspensions from normal mice very low, about 1/1000, is found mainly among large T cells and is completely dependent upon the presence of interleukin- 2 (IL-2). Lymph node T cells from mice that had been injected with antiIgD or infected with Nippostrongylus brasiliensis, treatments known to cause an IL-4-dependent increase in serum IgE, show a frequency of IL-4 producing cells five to ten times as great as T cells from naive donors. Although small resting T cells from naive donors have a frequency of IL-4 producing of about 1/2000, they can be "primed" in vitro to develop into IL-4 producing cells by culture with anti-CD3, IL-2 and IL-4; this increases the frequency of IL-4 producing cells to about 1/20. Both IL-2 and IL-4 are required for this in vitro priming method and interferon gamma fails to inhibit such priming. IL-4 is also made by long term mast cell lines in response to receptor cross-linkage, as are other members of the IL-4 family of lymphokines, notably IL-5, IL-3, and GM-CSF. Among normal spleen and bone marrow cells, a small population of cells have high affinity receptor for IgE. These Fcepsilon receptor positive cells have essentially all the IL-4 producing activity of spleen cells in response to high affinity IgE receptor cross- linkage; frequencies of IL-4 producing cells as high as 1/5 have been achieved. Many of these cells are Alcian Blue positive suggesting that they are either mast cells or basophils. Cells capable of forming mast cell colonies in methylcellulose have a frequency of about 1/10 in this cell population. On a per cell basis, the Fcepsilon receptor positive splenic cells appears to make more than 100-fold more IL-4 per cell than do cells from IL-3-dependent mast cell lines, suggesting that they may be important cells in the mediation of IL-4 dependent events in vivo.

白介素4（IL-4）是有效的淋巴因子，可调节多种多样 淋巴样和其他造血细胞的功能。 测量的测定 单细胞的IL-4产生表明已经开发出来。 它显示 T细胞能够在淋巴结细胞中产生IL-4的频率 正常小鼠的悬浮液非常低，约1/1000，主要是在 大的T细胞，完全取决于白介素的存在 2（IL-2）。 来自注射抗抗抗菌的小鼠的淋巴结T细胞 或感染了巴西龙骨术，已知会导致的治疗 血清IgE的IL-4依赖性增加，显示出产生IL-4的频率 细胞是幼稚供体的T细胞的五到十倍。 虽然 幼稚供体的小静息T细胞的频率为IL-4的频率 大约1/2000，可以在体外“底漆”成IL-4 用抗CD3，IL-2和IL-4培养细胞；这增加了 IL-4产生细胞的频率约为1/20。 IL-2和IL-4都是 这种体外启动方法和干扰素伽玛所必需的 抑制这种启动。 IL-4也是由长期肥大细胞系对受体制成的 交叉链接，以及IL-4家族的其他成员，淋巴细胞家族， 值得注意的是IL-5，IL-3和GM-CSF。 在正常的脾脏和骨髓细胞中， 少数细胞对IgE具有高亲和力受体。 这些 fcepsilon受体阳性细胞基本上具有所有IL-4产生 脾细胞的活性对高亲和力IgE受体交叉的活性 连锁; IL-4产生细胞的频率高达1/5 成就了。 这些细胞中有许多是alcian蓝色阳性，表明 它们要么是肥大细胞或嗜碱性细胞。 能够形成桅杆的细胞 甲基纤维素中的细胞菌落的频率约为1/10 细胞种群。 在每个细胞的基础上，fcepsilon受体阳性 脾细胞似乎比DO生产的IL-4超过100倍 来自IL-3依赖性肥大细胞系的细胞，表明它们可能是 在体内IL-4依赖性事件介导的重要细胞。