ANTIGEN RECOGNITION AND ACTIVATION OF IMMUNOCOMPETENT CELLS

免疫活性细胞的抗原识别和激活

• 批准号: 3809533
• 负责人: W E PAUL
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3809533
• 关键词: B cell receptor; B lymphocyte; T cell receptor; T lymphocyte; antibody receptor; biological signal transduction; cell differentiation; crosslink; fatty acylation; immunoglobulin E; interleukin 2; interleukin 3; interleukin 4; leukocyte activation /transformation; lymphokines; mast cell; membrane activity; membrane proteins; nucleic acid hybridization; phorbols; phosphorylation; protein biosynthesis; protein kinase C; proteins; receptor binding; receptor coupling; thymus; tissue /cell culture

Lymphokine production by cells of the immune system can be initiated by cross-linkage of cell surface receptors. This is particularly striking for T cells where the relevant receptors are the alpha/beta or gamma/delta cell receptors, for mast cells where the receptors involved are Fc(epsilon)Rl and Fc(gamma)RII, and for NK cells where the receptor involved is Fc(gamma)RIII. The production of lymphokines mediated in this way is strikingly modulated by the presence of lymphokines; interleukin-4 (IL-4) production by naive T cells is strikingly enhanced by IL-2 while IL-4 production by cells of the mast cell lineage has a requirement for IL-3. Mast cell production of IL-4 and of other lymphokines, such as IL-3, depends upon cross-linkage of Fc receptors. For lymphokine production in response to Fc(epsilon)Rl cross-linkage, there are several very interesting features. Lymphokine secretion can be detected within about 1 hr of stimulation, requires continued presence of the cross-linking stimulus and is inhibited by excessive cross-linkage. IL-3 and IL-4 production by these cells is markedly enhanced by pretreatment of the cells with high concentrations of IL-3. Low concentrations of IL-3 or IL-4, although they cause the cells to grow, do not prepare them to produce IL-3. Similar effects have been observed in normal cells; IL-4 production by Fc(epsilon)RI+ cells in response to receptor cross-linkage is strikingly enhanced by pretreatment with IL-3. The functions of IL-4 are mediated by binding to a high affinity receptor. The major molecular species that is cross-linked to membrane-bound (125)I-IL-4 is a 70,000 dalton molecule (p7O) while the major IL-4 binding molecules purified from cell lysates or from membrane-labelled cells have weights of 120,000 (pl2O) and of 40,000 (p4O). The former is the major form of the cell surface receptor; the latter appears to be a secreted form of the receptor. The nature of p7O has been enigmatic. We have shown that p7O has a peptide map, for (125)I-IL-4-bound peptides, similar to pl2O, implying that it is a fragment of pl2O but the conditions under which this fragment is generated and its physiologic significance remain to be established.

可以通过细胞的细胞产生免疫系统的淋巴因子来启动 细胞表面受体的交联。这特别惊人 相关受体是α/beta或γ/Delta细胞的T细胞 受体，涉及受体的肥大细胞是FC（Epsilon）RL 和FC（伽马）RII，对于所涉及的受体为的NK细胞 FC（伽马）RIII。以这种方式介导的淋巴细胞的产生是 淋巴细胞的存在引起了极大的调节。白介素4（IL-4） 幼稚T细胞的生产被IL-2显着增强，而IL-4则可以增强 肥大细胞谱系细胞的产生需要IL-3。 IL-4和其他淋巴细胞的肥大细胞产生，例如IL-3， 取决于FC受体的交联。用于淋巴因子的产生 对FC（Epsilon）RL交叉链接的响应，有几个非常有趣的 特征。可以在约1小时的时间内检测到淋巴动物分泌 刺激，需要持续存在交联刺激和 被过度的交叉链接抑制。 IL-3和IL-4生产 通过高较高的细胞预处理可显着增强细胞 IL-3的浓度。低浓度的IL-3或IL-4，尽管它们 导致细胞生长，不要准备它们产生IL-3。相似的 正常细胞已经观察到了影响。 IL-4生产 FC（Epsilon）Ri+细胞响应受体交叉链接，非常引人注目 通过IL-3预处理增强。 IL-4的功能是通过与高亲和力受体结合而介导的。 与膜结合的交联的主要分子物种 （125）I-IL-4是70,000道尔顿分子（P7O），而主要的IL-4结合 从细胞裂解物或膜标记的细胞中纯化的分子具有 重量为120,000（PL2O）和40,000（P4O）。前者是主要形式 细胞表面受体；后者似乎是一种分泌的形式 受体。 P7O的性质是神秘的。我们已经证明了P7O 具有（125）I-IL-4结合肽的肽图，类似于PL2O， 暗示它是PL2O的片段，但在此条件 产生碎片，其生理意义仍然是 已确立的。