RED CELL TYROSINE KINASES AND REGULATION OF METABOLISM

红细胞酪氨酸激酶和代谢调节

基本信息

批准号：
2180639
负责人：
PHILIP Stewart LOW
金额：
$ 15.15万
依托单位：
PURDUE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1988
资助国家：
美国
起止时间：
1988-12-01 至 1994-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2180639
关键词：
6 phosphofructokinase Mammalia aldehyde lyase cell growth regulation cell transformation enzyme activity enzyme induction /repression enzyme inhibitors enzyme mechanism enzyme substrate glycolysis phosphorylation protein tyrosine kinase reticulocytes

项目摘要

With the observation that tyrosine-protein kinases are commonly involved in cell growth control, significant effort has been devoted to understanding the identities, distributions and biochemical actions of these kinases. Such research has recently yielded the curious observation that elevated levels of tyrosine kinase activity are not only associated with rapidly growing and transformed cells, but in some cases also with quiescent, terminally differentiated cells. Since information on the roles of tyrosine kinases in nondividing cells might also provide insight into one or more aspects of their activities in transformed cells, we have undertaken an investigation of the regulation of the tyrosine kinase of erythrocytes and its influence on the properties of the major substrate band 3, especially on its ability to bind and regulate glycolytic enzymes. We intend to purify the above kinase and determine which endogenous/exogenous ligands modulate its activity. We also plan to determine whether activation/inhibition of the kinase in vivo regulates erythrocyte metabolism, as suggested from recent in vitro kinase in vivo regulates erythrocyte metabolism, as suggested from recent in vitro studies. Finally, because glycolytic rates and tyrosine kinase activity are often elevated in transformed cells, we plan to investigate the tyrosine phosphorylation of glycolytic enzyme binding proteins in nonerythroid cells in an attempt to understand the role they may play in the regulation of cell metabolism.

据观察，酪氨酸蛋白激酶通常涉及在细胞生长控制方面，人们付出了巨大的努力了解其身份、分布和生化作用这些激酶。此类研究最近产生了奇怪的观察结果酪氨酸激酶活性水平升高不仅与具有快速生长和转化的细胞，但在某些情况下也具有静止的、终末分化的细胞。由于有关信息酪氨酸激酶在非分裂细胞中的作用也可能提供见解为了研究它们在转化细胞中的活动的一个或多个方面，我们对酪氨酸激酶的调节进行了研究红细胞及其对主要特性的影响底物带 3，特别是其结合和调节的能力糖酵解酶。我们打算纯化上述激酶并测定哪些内源/外源配体调节其活性。我们还计划确定体内激酶的激活/抑制是否调节红细胞代谢，如最近体内体外激酶所示根据最近的体外实验表明，调节红细胞代谢研究。最后，因为糖酵解速率和酪氨酸激酶活性在转化细胞中经常升高，我们计划研究糖酵解酶结合蛋白的酪氨酸磷酸化非红系细胞试图了解它们可能发挥的作用细胞代谢的调节。