ACQUISITION OF GC/MS WITH CI/EI AND FAB SOURCES

使用 CI/EI 和 FAB 源获取 GC/MS

• 批准号: 3519264
• 负责人: LUIGI G. MARZILLI
• 金额: $ 30万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-02-01 至 1986-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3519264
• 关键词: chemical synthesis; drug metabolism; gas chromatography mass spectrometry; mass spectrometry

The current GC/MS facility available to the Emory community does not have adequate data handling facility, resolution, and mass range to deal with the series of biomedical projects currently funded. Recent advances in mass spectrometry have led to a new generation of commercial instruments which will make mass spectroscopy a much more important tool in biomedical research - in particular, high resolution magnetic sector systems which can be quickly recycled. This facility, combined with readily interchanged sources, offers the opportunity to investigate non-volatile biomolecules, drugs, etc. with wieghts greater than 1000 amu. Just as high field superconducting NMR instruments have been an integral part of biomedical research and are used extensively by non-NMR specialists, mass spectrometers of the type requested will gain wide acceptance in the near future. The biomedical research community at Emory has and will continue to expand and over half the major users have come to Emory only recently. The inadequate MS facilities are currently the greatest limitation we face in our research program. The requested instrument will serve a group of six major users. The department of chemistry alone will add two and possibly three additional faculty that will be users. A diverse set of research programs will be enhanced. These include: (a) the application of FAB/MS to the study of anti-cancer drug binding to nucleic acids; (b) the study of the distribution of fluorescent compounds in bacteria, (c) distribution of polynuclear aromatic compounds in oil refinery areas, (d) the preparation of labeled surfactants, (e) syntheses of antibiotics, (f) dipolar cycloaddition chemistry, (g) the synthesis of butenolide containing natural products, (h) oxidation-reduction enzyme mechanisms, and (i) organocobalt chemistry related to coenzyme B12 chemistry. If an award is made, competitive instruments from VG, Finnigan/MAT, and Kratos will be evaluated with our samples and by discussion with users of these instruments in academic institutions.

Emory社区可用的当前GC/MS设施没有 足够的数据处理设施，解决方案和质量范围来处理 目前资助的一系列生物医学项目。 最近的进步 质谱导致新一代商业仪器 这将使质谱学成为生物医学中更重要的工具 研究 - 特别是，高分辨率磁性扇形系统可以 快速回收。 这个设施，结合很容易互换 消息来源，提供了调查非易失性生物分子的机会， 毒品等，wieghts大于1000 AMU。 就像高场 超导NMR仪器一直是生物医学不可或缺的一部分 研究并被非NMR专家大量使用 所请求类型的光谱仪将在附近获得广泛接受 未来。 埃默里（Emory）的生物医学研究界已经并且将继续 扩展和一半以上的主要用户直到最近才来到Emory。 MS设施不足是我们面临的最大限制 在我们的研究计划中。 请求的仪器将为六个主要用户组成。 这 仅化学系将增加两个，可能再增加三个 将成为用户的教师。 各种各样的研究计划将是 增强。 其中包括：（a）Fab/MS在研究中的应用 抗癌药与核酸结合； （b）研究 细菌中荧光化合物的分布，（c）分布 炼油厂中的多核芳香化合物，（d）制剂 标记的表面活性剂，（e）抗生素的合成，（f）偶极 环加成化学，（g）含有天然的丁烯丁基的合成 产品，（H）氧化还原酶机制，（i）有机库 与辅酶B12化学有关的化学。 如果获得了奖励，则来自VG，Finnigan/MAT的竞争仪器以及 Kratos将通过我们的样本进行评估，并与 这些工具在学术机构中。