CORE--ANALYTICAL CORE

核心--分析核心

• 批准号: 6482471
• 负责人: AJAI K CHAUDHARY
• 金额: $ 14.08万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6482471
• 关键词: adduct; analytical chemistry; biomedical facility; blood chemistry; chemical structure; chemical synthesis; drug metabolism; electrospray ionization mass spectrometry; fast atom bombardment mass spectrometry; gas chromatography mass spectrometry; high performance liquid chromatography; mass spectrometry; statistics /biometry; urinalysis

In a multi-investigator, multi-project Program Project, a number of scientific and administrative support activities are required. These are best provided in a centralized fashion rather than attempting to fragment them according to a particular project and/or investigator, especially when there are many areas of overlap. The Support Core provides such centralized approaches and is based on two components. The analytical component will provide support to the various Projects for the structural, qualitative and quantitative analysis of drugs and metabolites. Gas chromatography/mass spectrometry analyses will be performed with high sensitivity an specificity by the use of stable isotope dilution methodology in combination with electron capture negative chemical ionization. Liquid chromatography/electrospray ionization tandem mass spectrometry will be employed for the identification of drug metabolites. Alternative mass spectrometry methodology that employs electron ionization, positive chemical ionization, liquid secondary ion, matrix-assisted laser desorption is available should it be required. This component will also provide a resource for the synthesis of drug metabolites and stable isotope analogs for use as standards for stable isotope dilution assays. Analytical procedures that require conventional HPLC methodology will be developed in the Core if they are required in the individual projects. An administrative component will provide the structure to support essential but non-scientifically related activities for the individual Projects. For example, administrative coordination, fiscal management, ordering of supplies, manuscript and grant-report preparation, and the provision of common services such as duplicating, services contracts, mailing and travel support, etc. In addition, biostatistical support for all of the Projects will be provided by the Core.

在多注射的多项目项目中，一个数字 需要科学和行政支持活动。 这些最好以集中的方式而不是 试图根据一个特定项目将它们分散 和/或调查员，尤其是在很多领域 重叠。 支持核心提供了这种集中的方法 并基于两个组件。 分析组件将 为结构性的各种项目提供支持， 药物和代谢产物的定性和定量分析。 气体 将进行色谱/质谱分析 具有高灵敏度，通过使用稳定同位素的特异性 稀释方法与电子捕获结合 负化学电离。 液相色谱/电喷雾 电离串联质谱法将用于 药物代谢产物的鉴定。 替代质谱 采用电子电离，阳性化学的方法论 电离，液体次级离子，基质辅助激光解吸为 如果需要可用。 此组件也将提供 用于合成药物代谢物和稳定同位素的资源 用作稳定同位素稀释测定标准的类似物。 需要常规HPLC的分析程序 如果需要在核心中开发方法 各个项目。 管理组件将提供 支持基本但无关的结构 各个项目的活动。 例如，管理 协调，财政管理，供应订购，手稿 和赠款报告的准备以及提供共同的 诸如复制，服务合同，邮寄和旅行等服务 支持等。此外，对所有的生物统计支持 项目将由核心提供。