PROTEIN SEQUENCER SYSTEM

蛋白质测序仪系统

• 批准号: 3521395
• 负责人: EDWARD BRESNICK
• 金额: $ 11万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-01 至 1993-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521395
• 关键词: aminoacid analyzer; biomedical equipment purchase; protein purification; protein sequence; synthetic peptide

This Shared Instrumentation Grant Application is for funds to purchase a peptide sequencer for a core laboratory at Dartmouth College and Dartmouth Medical School. The Protein Core Laboratory already contains a peptide synthesizer. Currently, all proteins must be forwarded to another university or industrial concern for sequencing which is expensive and not as efficient as if Dartmouth has its own instrument. A major user group consisting of nationally-funded investigators from the Departments of Biology, Biochemistry, Chemistry, Internal Medicine, Microbiology, Pharmacology and Toxicology, and Physiology has been identified. An Advisory Committee consisting of Drs. Bresnick, Ciardelli and Froehner has been appointed to provide groundrules for the use of the facility, to establish fees for service, and to assist when needed in priority setting for individual tasks (if asked by the Facility Director). The Advisory Committee has appointed Dr. Tom Ciardelli who will have the overall day-to-day responsibility for the protein sequencing service, will oversee the technician, and will establish priorities for proteins to be sequenced. The acquisition of the peptide sequencer will significantly improve the research posture of a group of peer-reviewed investigators and will accordingly, fill a void which is existent on the Dartmouth campus.

该共享的仪器赠款申请是为了购买 达特茅斯学院核心实验室的肽测序仪和 达特茅斯医学院。 蛋白质核心实验室已经包含 肽合成器。 目前，所有蛋白质都必须转发给 另一个大学或测序的工业问题是 昂贵的，不像达特茅斯有自己的工具那样高效。 一个 由全国资助的调查员组成的主要用户组 生物学，生物化学，化学，内科，内科， 微生物学，药理学和毒理学以及生理学一直是 确定。 由Drs组成的咨询委员会。布雷斯尼克（Ciardelli） Froehner已被任命为使用 设施，建立服务费，并在需要时协助 单个任务的优先设置（如果设施要求 导演）。 咨询委员会已任命汤姆·西亚德利（Tom Ciardelli）博士 将对蛋白质承担总体责任 测序服务，将监督技术人员，并将建立 测序蛋白质的优先级。 肽的获取 音序器将显着改善一组的研究姿势 经过同行评审的调查人员，将相应地填充一个空白 存在于达特茅斯校园。