Instrumentation for Peptide Synthesis Facility

肽合成设施仪器

• 批准号: 6577546
• 负责人: KATHRYN IVANETICH
• 金额: $ 24.02万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6577546
• 关键词: biomedical equipment purchase; high performance liquid chromatography; nucleic acid purification; nucleic acid sequence; protein purification; protein sequence

DESCRIPTION (provided by applicant): The Health Sciences campus of the University of California at San Francisco is one of the foremost centers of biomedical research in the world. Its Schools of Medicine, Pharmacy, Dentistry and Nursing employ several hundred outstanding investigators who have an exceptional record in achieving NIH awards. The UCSF campus is served by a state-of-the-art biotechnology core facility, the Biomolecular Resource Center (BRC). The BRC has served the UCSF campus since 1985, and fulfills investigators' needs in DNA and peptide sequencing, synthesis, analysis and purification. It is housed in 1,900 sq. ft. of laboratory/office space. Approximately 225 principal investigators have used the facility in the last year. Approximately 190 PIs have had 31,150 DNA templates sequenced, 105 PIs had ca. 3,100 oligonucleotides synthesized, over 30 PIs have had 155 peptides synthesized, and approximately 20 PIs had approximately 50 proteins sequenced. The facility is overseen by a Board of Overseers. Dr. Kathryn Ivanetich is the Director and oversees the day-to-day scientific, financial and administrative aspects of the BRC. Funding is requested for an Applied Biosystems 433A peptide synthesizer, Rainin Symphony Multiplex multiple peptide synthesizer, a Shimadzu analytical and preparative HPLC systems. The instruments are requested to replace outdated and obsolete instrumentation, and to provide increased automation to provide peptide synthesis, analysis and purification quickly, inexpensively and reliably to UCSF investigators. This request is supported by eleven UCSF Principal Investigators with considerable, ongoing PHS/NIH grant support. These PIs represent 8 UCSF departments, including Biochemistry and Biophysics, Medicine, Anatomy, Microbiology and Immunology, Physiology, Anesthesia, and Laboratory Medicine, plus the Cardiovascular Research Institute. The requested instrumentation would be a truly shared resource. No single investigator, group of investigators or department would dominate its use.

描述（由申请人提供）：加利福尼亚大学旧金山分校的健康科学校园是世界上最重要的生物医学研究中心之一。它的医学，药房，牙科和护理学校雇用了数百名杰出的调查员，他们在获得NIH奖项方面具有出色的记录。 UCSF校园由最先进的生物技术核心设施，生物分子资源中心（BRC）提供服务。 BRC自1985年以来一直为UCSF校园服务，并满足了研究人员在DNA和肽测序，合成，分析和纯化方面的需求。它位于实验室/办公空间的1,900平方英尺中。去年，大约有225名主要研究人员使用了该设施。大约有190个PI有31,150个DNA模板测序，有105个PIS有CA。 3,100个合成的寡核苷酸，超过30个PI已合成155肽，大约20个PI的测序约有50种蛋白质。该设施由监督委员会监督。 Kathryn Ivanetich博士是BRC的日常科学，财务和行政方面。要求资金用于应用生物系统433A肽合成器，Rainin Synin Multiplex多重肽合成器，Shimadzu分析和制备HPLC系统。要求这些工具代替过时的和过时的仪器，并提供增加的自动化以快速，廉价，可靠地向UCSF研究者提供肽合成，分析和纯化。该请求得到了11名UCSF首席调查员的支持，并提供了相当大的PHS/NIH赠款支持。这些PI代表8个UCSF部门，包括生物化学和生物物理学，医学，解剖学，微生物学和免疫学，生理学，麻醉和实验室医学，以及心血管研究所。请求的仪器将是真正共享的资源。没有一个调查员，研究人员或部门将主导其使用。