WOUND HEALING MODULATION IN GLAUCOMA FILTERING SURGERY

青光眼滤过性手术中的伤口愈合调节

基本信息

批准号：
3264709
负责人：
DAVID A LEE
金额：
$ 12.05万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
1988
资助国家：
美国
起止时间：
1988-08-01 至 1991-07-31
项目状态：
已结题

项目摘要

The primary objective of this project is to develop new methods for controlling wound healing following glaucoma filtration surgery (GFS) through the technology of polymeric delivery- controlled drug delivery. Bioerodible polymers will be used fot the localized and sustained of various drugs which inhibit scar tissue formation at the site of GFS in an animal model. A series of prospective, randomized, double-masked, and placebo- controlled experiments in an animal model will be performed by comparing a bioerodible polymer impregnated with a certain drug or drug combination to the same bioerodible polymer without any drug. All animals will undergo preoperative eye examinations including slit lamp biomicroscopy and pneumotonomery. A standard posterior lip sclerectomy will be performed on both eyes of each animal under general anesthesia. The polymer with drug will be randomly implanted in one eye and the fellow eye will receive the placebo polymer. Eye examinations will be performed one day after surgery and every other day thereafter for a minimum of five weeks or until the filtering surgery in the experimental eye fails. The parameters measured will include intraocular pressure, outflow facility, the appearance of the bleb, conjunctiva, polymer implant, cornea, and anterior chamber. At various times following surgery, histopathological examination will be performed on both experimental and control eyes. Quantitative morphology and immunohistochemistry will be used to assess the effect of drug delivery on scarring. This study will develop and refine the polymer characteristics of composition, dissolution rate, drug release rate, geometrical shape, and possible lamination with different drugs in order to improve its efficacy in GFS. An in vitro tissue culture system of fibroblasts will be developed to screen potentially useful drugs for delivery by the bioerodible polymers and to study the drug release characteristics of the bioerodible polymers impregnated with drugs. The in vitro fibroblastic growth will be measured by direct counting of fibroblasts and by using methyl-14C thymidine uptake. Using bioerodible polymers as a drug delivery device in GFS may dramatically improve the success of this procedure in patients at high risk for failure.

该项目的主要目标是开发新方法用于控制青光眼滤过后的伤口愈合通过聚合物输送技术进行手术（GFS）- 受控药物输送。可生物侵蚀的聚合物将用于各种抑制疤痕药物的局部和持续动物模型中 GFS 部位的组织形成。 A系列前瞻性、随机、双盲和安慰剂研究动物模型中的对照实验将由以下人员进行比较浸渍某种药物的生物可蚀性聚合物或与相同生物可蚀性聚合物的药物组合，没有任何药品。所有动物都将接受术前眼部检查包括裂隙灯生物显微镜和呼吸学。一个双眼将进行标准后唇巩膜切除术每只动物在全身麻醉下。含有药物的聚合物将随机植入一只眼睛，另一只眼睛将接受安慰剂聚合物。将进行眼科检查手术后一天，此后每隔一天进行一次至少五周或直到滤过手术实验眼失败了。测量的参数将包括眼压、流出设施、水泡的出现、结膜、聚合物植入物、角膜和前房。在术后多次、组织病理学检查将在实验眼和对照眼上进行。将使用定量形态学和免疫组织化学评估药物输送对疤痕的影响。这项研究将开发和完善组合物的聚合物特性，溶出速率、药物释放速率、几何形状和可能与不同药物层压以改善其 GFS 的功效。成纤维细胞体外组织培养系统将开发用于筛选潜在有用的药物递送通过生物可蚀性聚合物并研究药物释放浸渍的生物可蚀性聚合物的特性药物。体外成纤维细胞生长将通过直接测量成纤维细胞计数并使用甲基-14C胸苷摄取。在 GFS 中使用生物可蚀性聚合物作为药物输送装置可能显着提高患者的手术成功率失败的风险很高。