PHOSPHORIMAGING SYSTEM

磷光成像系统

基本信息

批准号：
2283879
负责人：
MICHAEL SOLURSH
金额：
$ 9.5万
依托单位：
UNIVERSITY OF IOWA
依托单位国家：
美国
项目类别：
财政年份：
1993
资助国家：
美国
起止时间：
1993-04-15 至 1994-10-14
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2283879
关键词：
biomedical equipment purchase biomedical equipment resource computer data analysis computer program /software computer system hardware image processing lasers photomultiplier

项目摘要

This is an application for funds to purchase a phosphorimager to be used in experiments vital to research being performed in the Departments of Biology and Botany at the University of Iowa. The equipment requested includes image plates, laser scanner, work stations, printer, tape and disk storage devices, and appropriate software for image processing and analysis. The proposed instrument will comprise an integrated system that will be housed in the Biology Electron Microscope Facility, which is a departmental facility available to all faculty, students and staff in the Departments of Biology and Botany. Over thirteen researchers involved in nineteen NIH-funded research grants will utilize the requested equipment for a wide range of biological applications ranging from quantitative analysis of DNA-protein interactions to determination of sites of gene expression to DNA sequence analysis. The primary advantage of the phosphor imager system is that it eliminates the need for conventional autoradiography, allowing marked increases in efficiency together with significant reductions in cost of materials and disposal of radioactivity and heavy metals associated with film processing. In addition to increasing efficiency, the increased sensitivity of the instrument will allow researchers to make new observations and develop new research directions. Support for purchase of the proposed instrument would allow for a type of analysis not currently present at the University of Iowa.

这是购买要使用的磷光成像仪的资金申请对各部门正在进行的研究至关重要的实验爱荷华大学生物学和植物学。要求的设备包括图像板、激光扫描仪、工作站、打印机、胶带和磁盘存储设备以及用于图像处理和处理的适当软件分析。拟议的仪器将包括一个集成系统它将被安置在生物电子显微镜设施中，是所有教职员工、学生和工作人员均可使用的院系设施在生物和植物学系。超过 13 名研究人员参与了 19 项 NIH 资助的研究资助将利用所需的设备进行广泛的生物应用范围从 DNA 蛋白质定量分析确定基因表达位点与 DNA 序列的相互作用分析。荧光成像仪系统的主要优点是它消除了传统放射自显影的需要，允许标记提高效率并显着降低成本与放射性和重金属相关的材料和处置胶片加工。除了提高效率之外，还增加了该仪器的灵敏度将使研究人员能够做出新的观察并开发新的研究方向。支持购买拟议文书将允许进行某种类型的分析，而不是目前就读于爱荷华大学。