FACTORS CONTROLLING PROTEIN SYNTHESIS

控制蛋白质合成的因素

• 批准号: 3269388
• 负责人: JOANNE M. RAVEL
• 金额: $ 10.79万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-04-01 至 1986-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3269388
• 关键词: Escherichia coli; Saccharomyces; bacterial genetics; cell differentiation; cell free system; density gradient ultracentrifugation; eukaryote; fungal genetics; gel filtration chromatography; genetic translation; messenger RNA; radiotracer; ribosomes; transfer RNA; wheat; yeasts

The overall objective of this proposed research is to elucidate the mechanism by which initiation of protein synthesis occurs in eukaryotic organisms. One of our objectives is to identify and characterize all of the factors required for the initiation of translation of mRNA in wheat germ, determine the function of these factors in the initiation process, and how these factors are functionally related to the mammalian initiation factors. A second objective is to identify the factors required for the initiation of protein synthesis on yeast mitochondrial ribosomes and determine how these factors are functionally related to eukaryotic cytoplasmic initiation factors and to Escherichia coli initiation factors.

这项研究的总体目标是通过以下方式阐明其机制： 蛋白质合成的起始发生在真核生物中。 之一 我们的目标是确定和描述所需的所有因素 小麦胚芽中 mRNA 翻译的起始，确定其功能 启动过程中的这些因素，以及这些因素如何发挥作用 与哺乳动物的起始因子有关。 第二个目标是确定 酵母中启动蛋白质合成所需的因素 线粒体核糖体并确定这些因素如何在功能上相关 真核细胞质起始因子和大肠杆菌起始因子 因素。