MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM

少数民族高中生研究学徒计划

• 批准号: 3512240
• 负责人: MARGARITA ORTIZ-MARCIALES
• 金额: $ 3万
• 依托单位: UNIVERSITY OF PUERTO RICO AT HUMACAO
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-03-01 至 1994-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3512240
• 关键词: minority institution research support

We are applying for the funds to purchase a much needed instrument, a Transmission Electron Microscope with the capabilities for distortion free imaging and high accelerating voltage for high contrast high magnification transmission electron microscopy. Utilizing immunoelectron microscopy and transmission electron microscopy aided morphometry of hearts, lungs and isolated cells we hope to gain new and useful information in the immunobiological reactions in the lung in response to oxygen toxicity, C5a induced inflammation and in hypertensive heart diseases. By utilizing immunoelectron microscopy we plan to study at molecular levels the assemblies of C5 convertase in the lung and the membrane attack complexes C5-9. Additionally, we will study the structural features of the cytolytic T cells and their interactions with the target tumor cells, as it pertains to their cytolytic granule content. These projects are NIH funded grants. Fulfillment of these research projects will be greatly enhanced by availability of the TEM requested. We also hope to obtain new information regarding the stoichiometry of C5-C5 convertase interactions and of the C5-C9 complex formation. Ultimate goal of all our research is to obtain data to provide useful information that can ultimately lead to development of preventive and therapeutic modes.

我们正在申请资金购买急需的 仪器，透射电子显微镜 无失真成像和高加速能力 高对比度高放大倍率传输电压 电子显微镜。 利用免疫电子显微镜和 透射电子显微镜辅助心脏形态测量， 我们希望获得新的和有用的肺部和分离的细胞 肺部免疫生物学反应的信息 对氧毒性、C5a 诱导的炎症的反应以及 高血压心脏病。 通过利用免疫电子 显微镜我们计划在分子水平上研究组件 肺中 C5 转化酶的变化和膜攻击 配合物C5-9。 此外，我们还将研究结构 溶细胞T细胞的特征及其与细胞的相互作用 靶向肿瘤细胞，因为它与其细胞溶解颗粒有关 内容。 这些项目是 NIH 资助的赠款。 实现这些 研究项目将因可用的 TEM 要求。 我们也希望获得新的信息 关于 C5-C5 转化酶相互作用的化学计量 以及C5-C9复合物的形成。 我们所有研究的最终目标是获取数据以提供 有用的信息最终可以导致开发 预防和治疗模式。