NUCLEAR TRANSFER IN NON-HUMAN PRIMATES

非人类灵长类动物的核转移

• 批准号: 3505292
• 负责人: STEVEN A VOELKEL
• 金额: $ 4.98万
• 依托单位: GRANADA BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-07-01 至 1991-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3505292
• 关键词: Primates; animal tissue; cell fusion; cell nucleus; cow; egg /ovum; electrostimulus; embryo /fetus; embryo /fetus culture

DESCRIPTION (Adapted from the applicant's abstract): Dr. Voelkel of Granada Biosciences requests Phase I funding to explore various methodologic factors preparatory to cloning non-human primate embryos. The limited availability of primates for research and the problems of disease within colonies suggest that cloned non-human primates would have substantial advantages for the research community. Presently, cloning techniques for a number of common eutherian mammals have been developed. The techniques are similar enough, between species, to assume that they can be successfully applied to primates with relatively little modification. Voelkel proposes to determine the timing of nuclear maturation in vitro, the ability of oocytes to activate in response to an electric stimulus, the most effective means of enucleating oocytes and the extent to which oocytes will fuse with non-primate blastomeres. He plans to obtain oocytes from animals at regional primate facilities that are being sacrificed for other purposes. The oocytes will be aspirated from secondary follicles and placed in various incubation media for transport to the investigators' laboratories. During Phase II the applicant intends to demonstrate the efficacy of the optimized techniques from Phase I to culture embryos.

描述（改编自申请人的摘要）： Granada Biosciences要求第一阶段资金探索各种 方法学因素为克隆非人类灵长类动物胚胎进行了准备。 这 灵长类动物的研究和疾病问题有限 在殖民地内表明，克隆的非人类灵长类动物会有 研究界的实质优势。 目前，克隆 已经开发了许多常见的Eutherian哺乳动物的技术。 这些技术在物种之间足够相似，可以假设它们可以 成功地应用于相对较少修改的灵长类动物。 Voelkel建议在体外确定核成熟的时机， 卵母细胞因电刺激而激活的能力， 最有效的诱形卵母细胞和卵母细胞的程度 将与非灵长类动物囊泡体融合。 他计划从 为其他地区牺牲的地区灵长类设施的动物 目的。 卵母细胞将从次级卵泡和 放置在各种孵化介质中，将其运输到研究人员 实验室。 在第二阶段，申请人打算证明 从I期到培养胚胎的优化技术的功效。