APPLIED EMBRYOLOGY TECHNIQUES IN NONHUMAN PRIMATES

胚胎学技术在非人类灵长类动物中的应用

• 批准号: 3499373
• 负责人: STEVEN A VOELKEL
• 金额: $ 5万
• 依托单位: HELIX INTERNATIONAL CORPORATION
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-30 至 1988-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3499373
• 关键词: biomedical equipment development; cryopreservation; dissection; early embryonic stage; embryo /fetus; embryo /fetus cell /tissue; embryo /fetus culture; embryo /fetus transplantation; mammalian embryology; microscopy; ovulation time; twin /multiplet

This project is to establish a colony of monozygotic non-human primate twins for biomedical research models and to develop the technology and in-house expertise for initiating work in inter- species embryo transfer in endangered primates. During the course of this research a nonsurgical embryo recovery technique which has been developed by the investigative team will be perfected for recovery of uterine stage embryos from rhesus monkey (Macaca mulatta) females. Recovered embryos (morula to blastocyst stages) will be microsurgically bisected into monozygotic twin demi-embryos (DE). The DE will be used to compare two culture systems (CMRL medium alone or with cocultured endometrial fibroblasts) for maintaining in vitro viability over 24 hours. Following culture, the DE will be stained with fluorescein diacetate to assess viability. The DE will then be fixed and stained to count the cell number or frozen for subsequent assessment of viability following cryopreservation. Ultimate success in producing a substantial colony of monozygotic non-human primate twins will provide uniquely valuable animal models for biomedical research.

该项目是建立一个单卵非人类的殖民地 生物医学研究模型的灵长类双胞胎，并开发 技术和内部专业知识，用于启动与 濒危灵长类动物中的物种胚胎转移。 在这项研究过程中，非外科胚胎恢复 调查团队开发的技术将 完善以从恒河猴中恢复子宫阶段胚胎 猴子（Macaca mulatta）女性。 回收的胚胎（莫拉 将对胚泡阶段）进行显微外科分配 单卵双胞胎Demi-Embryos（de）。 DE将习惯 比较两个培养系统（单独或单独或与 共培养的子宫内膜成纤维细胞）用于维持体外 24小时内的生存能力。 以下文化，DE将被染色 与荧光素二乙酸酯一起评估生存能力。 那将是 固定并染色以计算单元格数或冻结 随后评估冷冻保存后的生存能力。 产生大量的单卵菌群的最终成功 非人类的灵长类双胞胎将提供独特的有价值动物 生物医学研究模型。